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| 绍兴黄酒中ACE活性抑制肽的分离分析 | |
| 引用本文： | 戴军,陈尚卫,谢广发,刘俊,帅桂兰,顾小红,赵光螯.绍兴黄酒中ACE活性抑制肽的分离分析[J].分析测试学报,2006,25(4):74-77. |
| 作者姓名： | 戴军 陈尚卫 谢广发 刘俊 帅桂兰 顾小红 赵光螯 |
| 作者单位： | 1. 江南大学,分析测试中心,江苏,无锡,214036 2. 浙江古越龙山绍兴酒股份有限公司,浙江,绍兴,312000 3. 江南大学,生物工程学院,江苏,无锡,214036 |
| 基金项目： | 浙江省经贸委技术创新项目;浙江省绍兴市科技基金 |
| 摘 要： | 首次将绍兴黄酒中的肽类组分进行大孔吸附树脂柱层析和高效凝胶过滤色谱以及反相色谱的多步提取纯化与抑制血管紧张素转换酶（AcE）活性试验，并首次利用基体辅助激光解吸电离-飞行时间串联质谱分析和液相色谱-电喷雾电离四极杆-飞行时间串联质谱联用分析鉴定出黄酒中4种ACE活性抑制肽的氨基酸序列为：VEDGGV、PST、NT和LY。 |
| 关 键 词： | 黄酒 血管紧张素转换酶 活性肽 氨基酸序列 高效液相色谱 基体辅助激光解吸电离 串联飞行时间质谱 电喷雾电离 四极杆飞行时间串联质谱 |
| 文章编号： | 1004-4957（2006）04-0074-04 |
| 收稿时间： | 2005-10-28 |
| 修稿时间： | 2006-01-03 |
Isolation and Sequence Analysis of Angiotensin Converting Enzyme Inhibitory Peptides in Chinese Rice Wine |
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| DAI Jun,CHEN Shang-wei,XIE Guang-fa,LIU Jun,SHUAI Gui-lan,GU Xiao-hong,ZHAO Guang-ao.Isolation and Sequence Analysis of Angiotensin Converting Enzyme Inhibitory Peptides in Chinese Rice Wine[J].Journal of Instrumental Analysis,2006,25(4):74-77. | |
| Authors: | DAI Jun CHEN Shang-wei XIE Guang-fa LIU Jun SHUAI Gui-lan GU Xiao-hong ZHAO Guang-ao |
| Abstract: | Inhibitory peptides for angiotensin converting enzyme(ACE) were isolated from Chinese rice wine by hollow fibre ultrafiltration followed by column chromatography using AB-8 macroporous adsorption resins.Fractions of the peptides were purified on the TSK gel 2000 SWXL column using high performance gel filtration chromatography and on the symmetry C_(18) column using semi-preparative reverse-phase high performance liquid chromatography,respectively.Their ACE inhibitory activities were then determined.Amino acid sequences of four bioactive peptides identified by MALDI-TOF-TOF-MS and LC-ESI-Q-TOF-MS were VEDGGV,PST,NT and LY,respectively. |
| Keywords: | Chinese rice wine Angiotensin converting enzyme Bioactive peptides Amino acid sequences High performance liquid chromatography Matrix-assisted laser desorption/ionization Timeof-flight mass spectrometry Electrospray ionization Quadrupole-time of flight mass spectrometry |
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