基于聚合物点的电致化学发光生物传感器用于葡萄糖检测

羧基功能化的聚(9,9-二辛基芴基-2,7-二基)-co-(1,4-苯并-{2,1′,3}-噻二唑)]聚合物点(PFBT-COOH)在无外加共反应试剂的条件下具有高的电致化学发光(ECL)信号,且过氧化氢(H 2O 2)对其ECL具有高效猝灭作用。采用PFBT-COOH修饰玻碳电极,进一步交联葡萄糖氧化酶(GOD)以构建酶传感器(GOD/PFBT-COOH/GCE)。随着检测底液中葡萄糖浓度的增加,葡萄糖在GOD催化下原位产生的H2O2量增加,导致传感器的ECL信号逐渐减弱,从而实现葡萄糖的准确、快速、灵敏检测。此方法测得葡萄糖的线性范围为1.0×10^-7~3.0×10^-3 mol/L,检出限为3.0×10^-8 mol/L。血清样品中葡萄糖的加标回收率为98.5%~106%。该策略为酶传感器的构建提供了新思路,为葡萄糖的检测提供了新方法。

Detection of Glucose Using an Electrochemiluminescence Biosensor Based on Carboxyl Functionalized Polymer Dots

An electrochemiluminescence(ECL)biosensor based on carboxyl functionalized poly(9,9-dioctylfluorene-2,7-diyl)-co-(1,4-benzo-{2,1′-3}-thiadiazole)]polymer dots(PFBT-COOH)was constructed for the detection of glucose.The PFBT-COOH emitted a strong ECL signal without any exogenous coreactants,which could be effectively quenched by H 2O 2.In this work,PFBT-COOH was modified onto surface of glassy carbon electrode(GCE),and further cross-linked with glucose oxidase(GOD)to obtain the biosensor,GOD/PFBT-COOH/GCE.With increase of glucose concentration in detection solution,amount of H2O2 generated by glucose in situ under the catalysis of GOD would increase,leading to a gradual weakening of ECL signal at the biosensor,thus achieving an accurate,quick and sensitive detection of glucose.The linear range of glucose was in the range of 1.0×10^-7-3.0×10^-3 mol/L,and the detection limit was 3.0×10^-8 mol/L.The method was applid in the detection of glucose in serum samples with spiked recoveries of 98.5%-106%.Such a strategy provides a new idea for construction of enzyme sensor and a new method for glucose detection.