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| 牛奶中多种中性与酸性药物残留的高效液相色谱-串联质谱法同时测定 | |
| 引用本文： | 彭涛,陈冬东,代汉慧,李晓娟,李娜,丁双阳,江海洋,邓晓军.牛奶中多种中性与酸性药物残留的高效液相色谱-串联质谱法同时测定[J].分析测试学报,2010,29(7). |
| 作者姓名： | 彭涛 陈冬东 代汉慧 李晓娟 李娜 丁双阳 江海洋 邓晓军 |
| 作者单位： | 1. 中国检验检疫科学研究院,北京,100123 2. 中国农业大学,动物医学院,北京,100094 3. 上海出入境检验检疫局,上海,200135 |
| 基金项目： | 国家质检总局科技项目，上海市科委技术标准专项资助项目，中国检科院科研项目 |
| 摘 要： | 首次建立了牛奶中64种中性和酸性药物的高效液相色谱-串联质谱(HPLC-MS/MS)同时测定方法。样品中的残留药物用乙腈提取,经正己烷液-液分配除脂和HLB固相萃取柱净化后,以乙腈和甲酸-乙酸铵缓冲溶液为流动相,梯度洗脱,采用HPLC-MS/MS电喷雾正离子(ESI+)电离,多反应监测(MRM)模式检测,基质匹配外标法定量。方法的定量下限(LOQ)为1~100μg/kg,加标回收率为54%~100%,相对标准偏差小于12%。该方法操作简便,灵敏度、准确度、精密度均满足残留分析的要求。 |
| 关 键 词： | 高效液相色谱-串联质谱 中性和酸性药物 牛奶 残留 |
Simultaneous Determination of Multi-residue of Neutral and Acidic Drugs in Milk by High Performance Liquid Chromatography with Tandem Mass Spectrometer Detection |
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| PENG Tao,CHEN Dong-dong,DAI Han-hui,LI Xiao-juan,LI Na,DING Shuang-yang,JIANG Hai-yang,DENG Xiao-jun.Simultaneous Determination of Multi-residue of Neutral and Acidic Drugs in Milk by High Performance Liquid Chromatography with Tandem Mass Spectrometer Detection[J].Journal of Instrumental Analysis,2010,29(7). | |
| Authors: | PENG Tao CHEN Dong-dong DAI Han-hui LI Xiao-juan LI Na DING Shuang-yang JIANG Hai-yang DENG Xiao-jun |
| Institution: | PENG Tao1,CHEN Dong-dong1,DAI Han-hui1,LI Xiao-juan1,LI Na2,DING Shuang-yang2,JIANG Hai-yang2,DENG Xiao-jun3(1.Chinese Academy of Inspection and Quarantine,Beijing 100123,China,2.College of Veterinary Medicine,China Agricultural University,Beijing 100094,3.Shanghai Entry-Exit Inspection and Quarantine Bureau,Shanghai 200135,China) |
| Abstract: | A high performance liquid chromatography with tandem mass spectrometric(HPLC-MS/MS) method was firstly established for the simultaneous determination of multi-residue of 64 neutral and acidic drugs in milk.The samples were extracted with acetonitrile.The extracts were defatted with n-hexane by liquid-liquid partition,and purified by HLB solid-phase extraction cartridge.Acetonitrile and formic acid-ammonium acetate buffer were used as mobile phase by gradient elution.The analysis of target compounds was carr... |
| Keywords: | high performance liquid chromatography with tandem mass spectrometry neutral and acidic drugs milk residue |
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