中药材黄芪中有机氮农药残留量的液相色谱检测方法

研究了中药材黄芪中有机氮农药（包括涕灭威、呋喃丹、西维因、甲霜灵）残留量的液相色谱测定方法。采用混合溶剂提取，经Florisil及中性氧化铝柱层析净化后，再经Florisil小柱进一步净化及相转移，进入HyperOSD2C18柱分析，流动相为V（乙腈）∶V（水）=38∶62的混合液。检测波长为220nm，用外标法进行定量，当添加水平为0.07×10

Determination of Organonitrogen Pesticides Residue in Chinese Medicinal Crop Radix Astragali by High Performance Liquid Chromatography

A method for the determination of organonitrogen pesticides residue(including aldicarb, furadam, sevin and metalaxyl) in Radix Astragali by HPLC was investigated. Pesticides residue were extracted from samples with a mixed solvent. The extracts were cleaned up by chromatography with a 15 mm column filled with Florisil and neutral alumium oxide, and further cleaned up and phase _ transferred with a Florisil cartridge. A Hyper OSD2 C18 column was used in the analysis with a mobile phase of a acetonitrile－water solution(38∶62 by volume). The determination was performed with an external standard method at the detection wavelength of 220 nm. The relative standard deviations were 3.2％～7.1％, and the detection limits were 1.2×10－11～5.3×10－10. The recoveries were 75.4％～95.1％, and the correlation coefficients of linearity were more than 0.999 9.