五氯酚免疫层析检测试纸条的研究

利用胶体金免疫层析技术建立了一种快速检测五氯酚(PCP)残留的方法。采用柠檬酸三钠还原法制备大小一致、分布均匀、粒径为20 nm的胶体金颗粒,以此标记五氯酚抗体,制备金标抗体。将五氯酚包被抗原和羊抗鼠二抗分别结合于硝酸纤维膜上,依次将型号Millipore135硝酸纤维膜、型号VL78金标垫、型号SB06样品垫及吸水纸组装于PVC底板上,组装成胶体金免疫层析检测试纸条。通过试纸条上颜色的深浅,检测样品中PCP的残留量。试纸条检出限为10 ng/mL,检测时间为5 min。该方法检测所需试剂已预先包被在试纸条上,操作简单、重复性好、成本低廉,可用于五氯酚的现场快速检测。

Preparation of Colloidal Gold Immunochromatography Strip for Detection of Pentachlorophenol

A rapid method for detection of pentachlorophenol(PCP) residue was established based on gold immunochromatography assay.The colloidal gold strip for PCP was developed successfully.Colloidal gold particles with a size of 20 nm were prepared by trisodium citrate disoxidation method.PCP and anti-mouse goat immunoglobulins were jet-positioned onto a nitrocellulose membrane.Nitrocellulose membrane,gold jet pad,sample pad and absorbent paper were assembled and cut into detecting card,respectively.Through the strip color shades,the detection of PCP residue samples was performed.The detection limit of the colloidal gold strip was 10 ng/mL for PCP in 5 min,and that all needed reagents were included in the strip.This method is characteristic of simplicity,high sensitivity and low cost to achieve the rapid detection of PCP residue.