组织样品的直接质谱分析及其应用

质谱技术具有灵敏度高、分析速度快、能提供分子结构信息等特点,在生命科学研究领域扮演着重要角色,常用于组织样品分析。传统地,组织样品分析一般需经过研磨、萃取、分离等繁杂的预处理过程,导致耗时低效,无法满足大量样品高通量分析的实际需求,且不可避免地使组织样品中的一些活性成分损失。近年来,随着新型常压质谱技术的发展,使得组织样品可在无需样品预处理的条件下进行实时、非破坏、在线直接质谱分析,大大提高了分析效率。该文着重介绍了新型直接质谱技术在组织样品分析中的应用,并简要展望了该技术在生命科学、临床医学、食品科学、活体分析等领域的发展趋势。

Direct Mass Spectrometry Techniques and Their Applications in Tissue Samples Analysis

Ambient mass spectrometry has been increasingly applied in tissue sample analysis with the advantages of high sensitivity,high speed and the capability for providing molecular structure information of compounds in complex matrices.Traditionally,the time-consuming sample pretreatment procedures including homogenization,extraction and separation are inevitable for tissue sample analysis,and commonly associated with biological degradation,chemicals contamination and bioactive components loss in sample preparations.Recently,with the development of novel ambient mass spectrometry techniques,in situ tissue samples analysis has become possible without sample pretreatment. The applications of ambient mass spectrometry in tissue samples analysis are mainly discussed in this review,and their impacts in different fields, such as life science,clinical medicine,food science and in vivo analysis are prospected.