| 用反相高效液相色谱法分离和测定丁烯二酸的顺反异构体 |
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| 引用本文： | 李金昶,王广,崔秀君,赫奕,孙颜.用反相高效液相色谱法分离和测定丁烯二酸的顺反异构体[J].分析测试学报,2000,19(2):72-74. |
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| 作者姓名： | 李金昶 王广 崔秀君 赫奕 孙颜 |
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| 作者单位： | 1. 东北师范大学分析测试中心，吉林长春 130024
2. 吉林工业大学应用化学系，吉林长春 130025 |
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| 摘 要： | 建立了分离和测定丁烯二酸的２个异构体－顺丁烯二酸和反丁烯二酸的反相高效液相色谱法。以Ｓｈｉｍ－ｐａｃｋ ＣＬＣ－ＯＤＳ柱为分析柱，以甲醇－水（体积比５：２），用磷酸调至ｐＨ３．０）为流动相，流速０．７ｍＬ／ｍｉｎ，检测波长为２２０ｎｍ，外标法定量。顺、反丁烯二酸的检出限分别为０．１０和０．０７５ｍｇ／Ｌ（Ｓ／Ｎ＝２）；相对标准偏差分别为０．４７％和０．５４％（ｎ＝３）。二者的线性范围均为１０～６０
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| 关 键 词： | 高效液相色谱法 顺丁烯二酸 反丁烯二酸 异构体 |
| 文章编号： | 1004－4957（2000）02－0072－03 |
| 修稿时间： | 1999年5月30日 |
Determination of Two Butendioic Acid Isomers by Reversed phase High performance Liquid Chromatography |
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| LI Jin chang,WANG Guang,CUI Xiu jun,HE Yi,SUN Yan.Determination of Two Butendioic Acid Isomers by Reversed phase High performance Liquid Chromatography[J].Journal of Instrumental Analysis,2000,19(2):72-74. |
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| Authors: | LI Jin chang WANG Guang CUI Xiu jun HE Yi SUN Yan |
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| Institution: | LI Jin _ chang1,HE Yi2,SUN Yan2,WANG Guang1,CUI Xiu _ jun1 |
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| Abstract: | A method for the determination of two butendioic acid isomers by reversed phase high performance liquid chromatography is described. cis Butendioic and trans butendioic acids were separated by Shim－pack CLC－ODS column, using methanol－water(5∶2 by volume)solution adjusted to pH 3.0 by phosphoric acid as a mobile phase with flow rate of 0.7 mL/min. The quantitative results are obtained by external standard method. The detection wavelength of 220 nm is applied for the samples. The detection limits are 0.1 mg/L and 0.075 mg/L(S/N=2), and relative standard deviations are 0.47％ and 0.54％(n=3) for cis and trans butendioic acid, respectively. Linear range for both isomers is in 10～60 mg/L. The method is simple, rapid and sensitive, and was applied to determination of practical samples with satisfactory results. |
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| Keywords: | High _ performance liquid chromatography cis _ Butendioic acid trans _ Butendioic acid Isomer |
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