| 微波辅助提取/HPLC分析石蒜中的生物碱 |
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| 引用本文： | 范华均, 栾伟, 李攻科.微波辅助提取/HPLC分析石蒜中的生物碱[J].分析测试学报,2006,25(3):27-30. |
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| 作者姓名： | 范华均 栾伟 李攻科 |
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| 作者单位： | 1. 中山大学,化学与化学工程学院,广东,广州,510275;广东药学院,药科学院,广东,广州,510006
2. 中山大学,化学与化学工程学院,广东,广州,510275 |
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| 基金项目： | 国家自然科学基金;科技部科研项目 |
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| 摘 要： | 石蒜科石蒜属(amaryllidaceae)植物,含有石蒜碱、力可拉敏和加兰他敏(图1)等生物碱,具有镇静、镇痛、解热、降压、抗炎、催吐、抗病毒、抗癌等作用1]。目前石蒜生物碱的提取主要采用溶剂回流法(SRE),但SRE提取效率低、耗时、耗溶剂,而且有效成分易被破坏2-3]。微波辅助提取法
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| 关 键 词： | 微波辅助提取 HPLC 石蒜 石蒜碱 力可拉敏 加兰他敏 |
| 文章编号： | 1004-4957（2006）03-0027-04 |
| 收稿时间： | 2005-11-13 |
| 修稿时间： | 2006-03-02 |
Determination of Alkaloids in Lycoris radiata with Microwave-assisted Extraction Coupled with High Performance Liquid Chromatography |
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| FAN Hua-jun,LUAN Wei,LI Gong-ke.Determination of Alkaloids in Lycoris radiata with Microwave-assisted Extraction Coupled with High Performance Liquid Chromatography[J].Journal of Instrumental Analysis,2006,25(3):27-30. |
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| Authors: | FAN Hua-jun LUAN Wei LI Gong-ke |
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| Institution: | 1. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China; 2. College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China |
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| Abstract: | Lycorine,lycoramine and galanthamine in Lycoris radiata were microwave-assisted extracted(MAE) and determined by HPLC using triethylamine aqueous solution(pH 9.00)-acetonitrile-methanol as the mobile phase.The MAE operating conditions were optimized by the orthogonal test design combined with the mono-factor test.The linear ranges of the calibration curves for lycorine,lycoramine and galanthamine were 5.0-400 mg/L,respectively.Their detection limits were 5.0,5.1 and 10.2 ng,respectively and the RSD were 2.2%,5.7% and 4.4%,respectively.Compared with the results obtained by the conventional solvent reflux extraction method,the MAE was time saving and more efficient.The method was successfully applied to the extraction and determination of Lycorine,lycoramine and galanthamine in 10 Lycoris radiata samples from different regions. |
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| Keywords: | HPLC |
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