| LC-MS检测肝微粒体孵育液中系列化合物 |
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| 引用本文： | 王海龙,李劲彤,庄笑梅,李敬来,魏开华,阮金秀.LC-MS检测肝微粒体孵育液中系列化合物[J].分析测试学报,2004,23(Z1):70-71. |
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| 作者姓名： | 王海龙 李劲彤 庄笑梅 李敬来 魏开华 阮金秀 |
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| 作者单位： | 1. 军事医学科学院,国家生物医学分析中心,北京,100850
2. 军事医学科学院,毒物药物研究所,北京,100850 |
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| 基金项目： | 国家高技术研究发展课题资助项目(2003AA2Z3471) |
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| 摘 要： | 肝脏是最重要的药物代谢器官之一,肝微粒体孵育试验可在亚细胞水平确定药物代谢稳定性、药酶抑制、活性代谢物生成等重要特性。对孵育液中药物进行定性定量检测是一项关键性试验,本文利用液相色谱-离子阱质谱技术,成功地对肝微粒体孵育液中两个系列化合物进行了定性定量分析,探讨了样品前处理的重要影响因素。
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Determination of Series Compounds in Hepatic Microsome Incubating Solutions by LC-MS |
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| WANG Hai-long,LI Jin-long,ZHUANG Xiao-mei,LI Jing-lai,WEI Kai-hua,RUAN Jin-xiu.Determination of Series Compounds in Hepatic Microsome Incubating Solutions by LC-MS[J].Journal of Instrumental Analysis,2004,23(Z1):70-71. |
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| Authors: | WANG Hai-long LI Jin-long ZHUANG Xiao-mei LI Jing-lai WEI Kai-hua RUAN Jin-xiu |
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| Abstract: | Incubation with hepatic microsome solutions were the critical drug screening experiments. 2 series of compounds were incubated with microsome for investigating their metabolism stability and analyzed qualitatively and quantitatively by liquid chromatography coupled ion trap mass spectrometry. The method was sensitive, accurate and reliable. |
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| Keywords: | Ion trap mass spectrometry Hepatic microsome Qualitative analysis Quantitative analysis |
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