| 洛美沙星的铕离子荧光探针时间分辨荧光法测定 |
| |
| 引用本文： | 曹文丽,于永丽,徐淑坤,张秀娟.洛美沙星的铕离子荧光探针时间分辨荧光法测定[J].分析测试学报,2010,29(2). |
| |
| 作者姓名： | 曹文丽 于永丽 徐淑坤 张秀娟 |
| |
| 作者单位： | 东北大学,理学院,辽宁,沈阳,110004 |
| |
| 基金项目： | 辽宁省自然科学基金资助项目 |
| |
| 摘 要： | 采用时间分辨荧光法,以铕离子为荧光探针,依据Eu3+-La3+-LMX-SDS稀土共发光体系,建立了测定洛美沙星(LMX)含量的新方法。研究了配合物的紫外及荧光光谱,分析了配合物的发光机理,考察了溶液pH值、缓冲溶液种类及反应试剂加入量对配合物荧光强度的影响。在优化的实验条件下,配合物荧光强度和洛美沙星浓度呈线性关系,相关系数为0.999 0,线性范围为5.0×10-7~1.0×10-5mol/L。方法的检出限为6.8×10-8mol/L,对5.0×10-6mol/L的洛美沙星溶液测定11次,RSD为0.6%。将该方法用于尿液中洛美沙星含量的测定,回收率为94%~104%。
|
| 关 键 词： | 铕 洛美沙星 荧光探针 时间分辨荧光法 |
Determination of Lomefloxacin Using Europium Ion as Fluorescent Probe by Time-resolved Fluorometry |
| |
| CAO Wen-li,YU Yong-li,XU Shu-kun,ZHANG Xiu-juan.Determination of Lomefloxacin Using Europium Ion as Fluorescent Probe by Time-resolved Fluorometry[J].Journal of Instrumental Analysis,2010,29(2). |
| |
| Authors: | CAO Wen-li YU Yong-li XU Shu-kun ZHANG Xiu-juan |
| |
| Institution: | CAO Wen-li,YU Yong-li,XU Shu-kun,ZHANG Xiu-juan(College of Science,Northeastern University,Shenyang 110004,China) |
| |
| Abstract: | A time-resolved fluorometric method for the determination of lomefloxacin(LMX) based on columinescence effect of Eu3+-La3+-LMX-SDS(sodium dodecyl sulfate) system was developed using europium ion as fluorescent probe.UV and fluorescence spectra of the complexes were studied and the luminescence mechanism was discussed.In the complex of Eu3+-LMX-SDS,LMX absorbs UV light at 283 nm and transfers the energy to Eu3+,that is,intramolecular energy transfer occurring.In pH 6.8 hexamethylene tetramine(HMTA)-HCl buffe... |
| |
| Keywords: | europium lomefloxacin fluorescent probe time-resolved fluorometry |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
