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苦荞叶片中芸香甙的薄层色谱定量研究
引用本文:张政,冯育林.苦荞叶片中芸香甙的薄层色谱定量研究[J].分析测试学报,1995,14(2):62-65.
作者姓名:张政  冯育林
作者单位:山西大学分析测试中心
摘    要:根据芸香甙的理化性质,建立了一种测定植物样品中芸香甙含量的薄层紫外扫描法,采用硅胶GF254薄层板,样品经甲醇提取浓缩后点样,展开剂为乙酸乙酯-甲酸-水(8+1+1)。用薄层色谱扫描仪在波长355nm处测定芸香甙的吸收强度并直接定量。该法操作简便,灵敏度高,定量准确,结果稳定,应用于苦荞叶片中芸香甙含量测定,得到良好结果,线性范围为0~2.0μg/μL,斑点的最低检出限为5.0ng,回收率102%

关 键 词:芸香甙  苦荞  薄层色谱

Determination of Rutin in Tartary Buckwheat Leaves by Thin-layer Scanning Technique
Zhang Zheng,Feng Yulin,Xie Shujing.Determination of Rutin in Tartary Buckwheat Leaves by Thin-layer Scanning Technique[J].Journal of Instrumental Analysis,1995,14(2):62-65.
Authors:Zhang Zheng  Feng Yulin  Xie Shujing
Abstract:Samples were extracted with methanol and applied to silica gel G TLC plate and developed with eluent composed of ethyl acetate-methanol-water(8 1 1).The developed plate was scanned with a TLC scanner zigzag mode at 355nm, Rutin in tartary buckwheat leaves have been determined by this technique with satisfactory results.The detection limit and recovery of the method are 5.0ng/spot and 102% respectively.
Keywords:Rutin  Tartary buckwheat leaves  TLC-scanner    
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