电子显微技术应用于生物纳米材料表征与测试的研究进展

该文简述了电子显微技术的发展历程,并介绍了现代电子显微镜的新功能。针对生物纳米材料理化性能与功能应用的特殊性,结合研究实例,重点阐述运用电子显微结构表征与原位分析测试技术指导构建新颖纳米结构、揭示材料与细胞/组织相互作用并发挥功能的机制。并在此基础上,展望了电子显微技术在生物纳米材料研究领域的发展方向(大尺寸图像拼接、三维重构、动态原位实时成像)。

Research Progress on Applications of Electron Microscopy in Characterization and Analysis of Bio-nanomaterials

The development course of electron microscopy(EM) and its new functions in recent years are introduced in this article.In bio nanomaterials research,EM could be employed to carry out morphology characterization and in situ chemical analysis,but higher standards of experimental methods are necessary because of the diversity of biomaterials,the complexity of newly created nanostructures and their functionalities tightly connected with living cells/organs.Therefore,the strategies to develop advanced EM hardware and software required by these specialties of bio nanomaterials are systematically explained.Besides,the development directions for EM in field of bio nanomaterials research such as large size mosaic image with high resolution,three dimensional modeling of tiny and complicate structures,real time and in situ characterization and analysis during experiments are prospected.