基于特征碎裂片段的葛根保健品中功效成分非定向筛查方法构建

通过超高效液相色谱-四极杆-静电场轨道阱高分辨质谱联用(UPLC-Q-Orbitrap HRMS),采用数据依赖采集模式对葛根保健品中15种功效成分的标准品进行分析,建立相应的标准谱库;对功效成分的断裂机理进行研究,得到其特征碎裂片段,并以数据非依赖型采集模式快速筛查葛根保健品中已知和未知的功效成分。15种功效成分在相应质量浓度范围内线性关系良好,相关系数(r~2)均大于0.99;不同加标水平下的回收率为83%～110%,相对标准偏差(RSD)为2.1%～8.6%。将该方法应用于实际样品分析,共筛查到16种功效成分,其中4种为所建标准谱库外的功效成分,表明所建方法可用于葛根保健品中功效成分的筛查。

Non-directive Screening for Functional Components in Food Supplements of Pueraria Based on Characteristic Fragmentation

An ultra performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry(UPLC-Q-Orbitrap HRMS) with data dependent acquisition mode was developed for the analysis of 15 functional ingredients in food supplements of pueraria,and a corresponding standard library was also established.By studying the fragmentation mechanism of the functional ingredients,the fragments during fragmentation were obtained.Data-independent acquisition mode was used for the rapid screening of known and unknown functional ingredients in food supplements of pueraria.There existed good linear relationships for 15 functional components in corresponding concentration ranges with correlation coefficients(r2) larger than 0.99.The recoveries at different spiked levels ranged from 83% to 110%,with relative standard deviations(RSD) of 2.1%-8.6%.The established method was applied in actual sample analysis,in which 16 components were screened out,and 4 of them were components beyond the established standard library.Results indicated that the method was feasible in the screening of functional components in food supplements of pueraria.