首页 | 本学科首页   官方微博 | 高级检索  
     检索      

液相色谱串联质谱法同时测定饲料中8种苯并咪唑类药物
引用本文:吴银良,皇甫伟国,杨挺.液相色谱串联质谱法同时测定饲料中8种苯并咪唑类药物[J].分析测试学报,2010,29(12).
作者姓名:吴银良  皇甫伟国  杨挺
作者单位:农业部农产品质量安全监督检验测试中心(宁波);
基金项目:宁波市自然科学基金资助项目,宁波市农科院院长基金资助项目
摘    要:建立了同时测定饲料中8种苯并咪唑类药物(噻苯咪唑、丙硫咪唑、硫苯咪唑、苯硫氧咪唑、氟苯咪唑、甲苯咪唑、丙氧苯唑和三氯苯唑)的液相色谱串联质谱分析方法。饲料样品用酸化乙腈直接提取,提取液用甲酸溶液稀释后进行分析。分析时用XBridgeTMC18色谱柱,以甲酸溶液-乙腈体系进行梯度洗脱,MRM方式测定,基质外标法定量。8种苯并咪唑类药物均在0.02~10.0 mg.L-1范围内呈良好的线性关系,相关系数(r2)均不低于0.990,在饲料样品中的检出限为2.1~63.0μg.kg-1。饲料中苯并咪唑类药物在0.50、30、200 mg.kg-13种加标水平下的回收率为84%~104%,相对标准偏差均小于10.0%。方法分析单个样品约需30 min,该方法适合饲料中8种苯并咪唑类药物的同时分析。

关 键 词:苯并咪唑类药物  液相色谱串联质谱法  饲料

Simultaneous Determination of Eight Benzimidazoles in Animal Feed by Liquid Chromatography-Tandem Mass Spectrometry
WU Yin-liang,HUANGFU Wei-guo,YANG Ting.Simultaneous Determination of Eight Benzimidazoles in Animal Feed by Liquid Chromatography-Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2010,29(12).
Authors:WU Yin-liang  HUANGFU Wei-guo  YANG Ting
Institution:WU Yin-liang,HUANGFU Wei-guo,YANG Ting(The Supervision,Inspection & Testing Center of Agricultural Products Quality & Security,Ministry of Agriculture(Ningbo),Ningbo 315040,China)
Abstract:A high performance liquid chromatography-tandem mass spectrometric(LC-MS/MS) method was developed for the determination of benzimidazoles,e.g.thiabendazole,oxfendazole,oxibendazole,mebendazole,fenbendazole,flubendazole,albendazole and triclabendazole,in animal feed.The samples were extracted with 0.5% formic acid in acetonitrile by ultrasonic extraction.The extract was analyzed by LC-MS/MS on an XBridgeTM C18 column with a mixture of 0.1% formic acid(A) and acetonitrile(B) as mobile phase.The mass spectrome...
Keywords:benzimidazoles  liquid chromatography with tandem mass spectrometry  animal feed  
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号