利巴韦林原位聚合分子印迹电化学传感器的研制

利用原位聚合分子印迹技术,以3-氨基苯硼酸(3-ABBA)为功能单体,利巴韦林(RIB)为目标分子,以硼酸和顺式二醇在不同酸碱度条件下可逆形成环内酯键为原理,在玻碳电极表面原位聚合形成利巴韦林分子印迹膜,研制了测定利巴韦林的分子印迹电化学传感器。采用循环伏安法(CV)和差分脉冲法(DPV)对印迹膜性能进行研究。DPV测试表明:在最优实验条件下,利巴韦林的浓度在5.0×10~(-8)~1.0×10~(-5)mol/L范围内与峰电流呈良好的线性关系,相关系数(r~2)为0.995 3,检出限(S/N=3)为1.5×10~(-8)mol/L。特异性实验表明制备的传感器对利巴韦林的选择性良好。该分子印迹电化学传感器可用于食品中利巴韦林的检测。

Development of an Electrochemical Sensor for Detection of Ribavirin Based on In-situ Polymerization Molecularly Imprinted Film

A novel electrochemical sensor for the determination of ribavirin (RIB) was fabricated by molecularly imprinted technology,based on 3-aminobenzeneboronic acid as functional monomer,and ribavirin as target molecule.A molecularly imprinted polymers(MIPs) membrane was synthesized on the surface of glassy carbon electrode in vacuum drying oven,by the formation principle of ring ester bond with boric acid and vicinal diol.The electrochemical behavior of the membrane was characterized by cyclic voltammetry(CV) and differential pulse voltammetry(DPV).Under the optimum conditions,the response of peak currents was linear to concentration of RIB in the range of 5.0 × 10-8-1.0 × 10-5 mol/L with a detection limit(S/N =3) of 1.5 × 10-8 mnol/L.The electrochemical sensor has the advantages of high sensitivity,good stability and high reproducibility,and could be uesd to detect RIB in food.