QuEChERS/超高效液相色谱-串联质谱法测定果蔬中122种香港规例农药残留

应用改进的QuEChERS法结合超高效液相色谱-串联质谱仪,建立了水果、蔬菜中122种中国香港《食物内除害剂残余规例》农药残留的测定方法。分析物采用电喷雾离子源,正、负离子多反应监测(MRM)模式,基质匹配外标法定量。122种农药在0.010~0.20 mg/L浓度范围内呈良好线性关系,其线性相关系数(r)不小于0.99,方法的定量下限为0.010 mg/kg;3个加标水平的回收率为60%~110%;相对标准偏差(RSD)为1.8%~19.4%。该法快速简便,耗时短,灵敏度高,稳定性好,用于日常供港果蔬食品中农残检测可显著降低检测成本。

Determination of 122 Pesticide Residues in Fruits and Vegetables from Regulation of Pesticides in Hong Kong by QuEChERS Combined with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

A method for the determination of 122 pesticide residues in fruits and vegetables from the Hong Kong's regulation was developed by QuEChERS combined with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The quantification and confirmation was achieved by electrospray ionization (ESI) in positive and negative ion mode using multiple reaction monitoring (MRM) mode,and the analytes were quantified by the external standard method.The standard curves of 122 target compounds were linear in the range of 0.010-0.20 mg/L,with correlation coefficients(r) not less than 0.99.The limits of quantitation(LOQ) were 0.010 mg/kg.The recoveries at three spiked levels were in the range of 60％-110％ with RSDs of 1.8％ and 19.4％.The method had the advantages of rapidness,simplicity,time-saving,high sensitivity and good stability,and could significantly reduce the test cost.