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鱼翅中硫酸软骨素的酶解-高效液相色谱法测定
引用本文:鲍伦军,杨建成,何振华,杨晓云,梁伟大.鱼翅中硫酸软骨素的酶解-高效液相色谱法测定[J].分析测试学报,2002,21(5):59-61.
作者姓名:鲍伦军  杨建成  何振华  杨晓云  梁伟大
作者单位:1. 广州出入境检验检疫局食品实验室,广东广州,510623
2. 江门出入境检验检疫局,广东江门,529051
3. 华南农业大学资源环境学院,广东广州,510640
基金项目:广东出入境检验检疫局基金资助项目(GDK9903K)
摘    要:建立了一种测定鱼翅中硫酸软骨素的酶解-高效液相色谱方法;该法利用在一定条件下硫酸软骨素能被硫酸软骨素酶ABC定量酶解成软骨素二糖,通过高效液相色谱法测定软骨素二糖的量来计算硫酸软骨素的含量。

关 键 词:鱼翅  硫酸软骨素  高效液相色谱法  酶解  产品分析
文章编号:1004-4957(2002)05-0059-03
修稿时间:2002年3月5日

Determination of Chondroitin Sulfate in Shark's Fins by Enzymolysis- HPLC
BAO Lun-jun,YANG Jian-cheng,HE Zheng-hua,YANG Xiao-yun,LIANG Wei-da.Determination of Chondroitin Sulfate in Shark''s Fins by Enzymolysis- HPLC[J].Journal of Instrumental Analysis,2002,21(5):59-61.
Authors:BAO Lun-jun  YANG Jian-cheng  HE Zheng-hua  YANG Xiao-yun  LIANG Wei-da
Institution:BAO Lun-jun1,YANG Jian-cheng2,HE Zheng-hua2,YANG Xiao-yun3,LIANG Wei-da1
Abstract:A method for the determination of chondroitin sulfate in shark's fins is described by enzymolysis-HPLC. On certain conditions chondroitin sulfate in shark's fins can be quantitatively converted to chondroitin disaccharide by enzymolysis of chondroitinase ABC, then the chondroitin disaccharide was determined by HPLC with a UV detector at 228 nm. The content of chondroitin sulfate in shark's fins can be calculated by quantitative relation between chondroitin sulfate and chondroitin disaccharide. This method was applied to the analysis of marketing shark's fins with satisfactory results.
Keywords:Shark's fins  Chondroitin sulfate  HPLC  Enzymolysis
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