| 气相色谱串联四极杆质谱法对富硒包菜汁中硒甲基硒半胱氨酸与硒蛋氨酸的测定 |
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| 引用本文： | 吴胜芳,莫海珍,王利平,刘杨岷.气相色谱串联四极杆质谱法对富硒包菜汁中硒甲基硒半胱氨酸与硒蛋氨酸的测定[J].分析测试学报,2008,27(12). |
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| 作者姓名： | 吴胜芳 莫海珍 王利平 刘杨岷 |
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| 作者单位： | 1. 江南大学,食品科学与技术国家重点实验室,江苏,无锡,214122
2. 河南科技学院,食品学院,河南,新乡,453003 |
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| 基金项目： | 上海市科学技术委员会专项基金重点项目，上海交通大学青年基金 |
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| 摘 要： | 建立了一种快速分析富硒包菜汁中硒甲基硒半胱氨酸和硒蛋氨酸含量的方法。以氯甲酸乙酯作衍生化试剂,利用串联质谱技术在多离子监测模式下对衍生物进行GC-MS/MS测定。方法的线性范围为0.01～25 mg/L,线性相关系数r≥0.9988;方法检出限分别为硒甲基硒半胱氨酸（met-Se-cys）4μg/L、硒蛋氨酸（Se-met）2μg/L;met-Se-cys的方法回收率为82%～97%,RSD小于6.3%,Se-met的方法回收率为100%～109%,RSD小于7.5%。结果表明该方法简单、快速、准确,并能有效排除基质的干扰,适合于食品中硒甲基硒半胱氨酸和硒蛋氨酸的定性定量分析。
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| 关 键 词： | 包菜 四极杆串联质谱 衍生化 硒甲基硒半胱氨酸 硒蛋氨酸 |
Determination of Se-methylselenocysteine and Selenomethionine in Selenium-enriched Cabbage by Gas Chromatography-Tandem Quadrupole Mass Spectrometry |
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| WU Sheng-fang,MO Hai-zhen,WANG Li-ping,LIU Yang-min.Determination of Se-methylselenocysteine and Selenomethionine in Selenium-enriched Cabbage by Gas Chromatography-Tandem Quadrupole Mass Spectrometry[J].Journal of Instrumental Analysis,2008,27(12). |
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| Authors: | WU Sheng-fang MO Hai-zhen WANG Li-ping LIU Yang-min |
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| Abstract: | A rapid GC-MS/MS method has been developed for the determination of Se-methylselenocysteine(met-Se-cys)and selenomethionine(Se-met)in selenium-enriched cabbage by using ethyl chloroformate(ECF)as a derivation reagent under multiple reaction monitor(MRM) mode.Under the optimal conditions,the samples were analyzed by using ornithine as an internal standard.The calibration curve showed good linear range of 0.01-25 mg/L with correlation coefficients of more than 0.998 8.The detection limits of met-Se-cys and Se... |
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| Keywords: | cabbage tandem quadrupole mass spectrometry derivation met-Se-cys Se-met |
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