| 禽蛋中头孢噻肟残留的高效液相色谱-串联质谱法测定 |
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| 引用本文： | 罗辉泰,黄晓兰,吴惠勤,朱志鑫,黄芳,林晓珊.禽蛋中头孢噻肟残留的高效液相色谱-串联质谱法测定[J].分析测试学报,2010,29(8). |
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| 作者姓名： | 罗辉泰 黄晓兰 吴惠勤 朱志鑫 黄芳 林晓珊 |
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| 作者单位： | 中国广州分析测试中心,广东省分析测试技术公共实验室,广东,广州,510070 |
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| 摘 要： | 建立了高效液相色谱-串联质谱(LC-MS/MS)测定禽蛋中头孢噻肟药物残留的方法。禽蛋样品中的头孢噻肟用纯水提取,乙腈沉淀蛋白,Oasis HLB(500 mg,6 mL)固相萃取柱净化,8 mL甲醇洗脱。采用Zorbax XDB-C18(2.1 mm×50 mm,3.5μm)色谱柱,以0.2%甲酸水-乙腈为流动相,0.3 mL/min梯度洗脱,经高效液相色谱分离后,采用电喷雾质谱正离子模式电离,多反应选择离子检测(MRM)模式测定。检测离子对为m/z456.1/396.1、m/z456.1/324.1,其中m/z456.1/396.1为定量离子对。在1.35~135μg/L范围内标准曲线的线性关系良好,相关系数为0.999 3;在1.0、50.0、100μg/kg3个添加水平的平均加标回收率为87%~99%,相对标准偏差为1.9%~3.9%;方法检出限为0.3μg/kg,定量下限为1.0μg/kg。该方法简便、灵敏、准确、可靠,适用于禽蛋中头孢噻肟药物残留的分析。
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| 关 键 词： | 头孢噻肟 高效液相色谱 串联质谱 禽蛋 残留 |
Determination of Cefotaxime Residue in Poultry Egg by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| LUO Hui-tai,HUANG Xiao-lan,WU Hui-qin,ZHU Zhi-xin,HUANG Fang,LIN Xiao-shan.Determination of Cefotaxime Residue in Poultry Egg by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2010,29(8). |
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| Authors: | LUO Hui-tai HUANG Xiao-lan WU Hui-qin ZHU Zhi-xin HUANG Fang LIN Xiao-shan |
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| Institution: | LUO Hui-tai,HUANG Xiao-lan,WU Hui-qin,ZHU Zhi-xin,HUANG Fang,LIN Xiao-shan(Guangdong Provincial Public Laboratory of Analysis and Testing Technology,China National Analytical Center(Guangzhou),Guangzhou 510070,China) |
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| Abstract: | A confirmative method to determine cefotaxime residue in poultry egg by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) under multiple reaction monitoring(MRM)mode was developed.The cefotaxime in sample was extracted with water.Sample preparation involved the protein precipitation with acetonitrile and the clean-up on an Oasis HLB(500 mg,6 mL) solid phase extraction(SPE) cartridge.The analyte was eluted from the SPE cartridge with 8 mL methanol.The separation of cefotaxime was pe... |
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| Keywords: | cefotaxime high performance liquid chromatography(HPLC) tandem mass spectrometry(MS/MS) poultry egg residue |
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