氧化铜-过氧化聚吡咯-还原氧化石墨烯复合膜修饰电极非酶检测葡萄糖

先以氧化石墨烯(Graphen oxide,GO)为阴离子掺杂剂,采用电化学聚合法制备了聚吡咯-氧化石墨烯复合膜(PPy-GO)。分别在0.10 mol/L Na Cl和0.10 mol/L NaOH溶液中对其进行还原和过氧化处理,制得过氧化聚吡咯-还原氧化石墨烯复合膜(OPPy-ERGO)。再以此OPPy-ERGO复合膜为载体,采用电化学沉积法制备了氧化铜-过氧化聚吡咯-还原氧化石墨烯复合膜修饰电极(CuO-OPPy-ERGO/CCE)。通过扫描电镜和电化学方法对此电极进行表征,研究了葡萄糖在此修饰电极上的电化学行为。结果表明,此电极对葡萄糖的电氧化过程表现出高的催化活性和良好的抗干扰能力。在0.20 mol/L NaOH溶液中,安培法检测葡萄糖的线性范围为5.0×10~(-7)~1.0×10~(-3)mol/L,检出限(3Sb)为2.0×10~(-7)mol/L,灵敏度为121.8μA/(mmol·L~(-1))。该电极用于血清中葡萄糖含量的测定,加标回收率为96.0%~110.1%。

Non-enzymatic Detection of Glucose Using a Copper Oxide-Overoxidized Polypyrrole-Reduced Graphene Oxide Composite Film Modified Electrode

A copper oxide-overoxidized polypyrrole-reduced graphene oxide composite film(CuO-OPPy-ERGO/CCE) modified electrode was fabricated by using layer by layer electrochemical deposition technique.Firstly,a polypyrrole (PPy)-graphene oxide (GO) composite film was prepared by electropolymerization of pyrrole while using GO as the anionic dopant.After the electrochemical reduction in O.10 moL/L NaCl solution and peroxidation in 0.10 mol/L NaOH solution,an overoxizied polypyrrole-reduced graphene oxide(OPPy-ERGO) composite film was obtained.Then,copper oxide was electrochemically deposited on this OPPy-ERGO film,and the modified electrode was obtained.The surface morphology and the electrochemical behavior of this modified electrode were investigated by scanning electron microscopy(SEM) and cyclic voltammetry.Furthermore,the electrochemical behavior of glucose on this electrode was also investigated carefully.The results showed that this modified electrode exhibited a high electrocatalytic activity and a good anti-interference ability for the oxidation of glucose.Under the optimum conditions,the linear range for the determination of glucose by amperommetry was in the range of 5.0 × 10-7-1.0 × 10-3 mol/L.The detection limit(3Sb =3) and the sensitivity was 2.0 × 10-7 mol/L and 121.8 μA/(mmol · L-1),respectively.The modified electrode was successfully used to determine glucose in human serum sample with recoveries of 96.0％-110.1％.