海洋真菌Hypoxylor oceanicum对1,2,3,4-四氢萘的转化及其产物测定

南海海洋真菌Hypoxylon oceanicum(326#)以葡萄糖(10g／L)、蛋白胨(2g／L)、酵母膏(1g／L)、20％的人工海水为培养基，在培养液中添加200mg／L的1，2，3，4-四氢萘，培养7d，对转化产物进行提取，经GC—MS和NMR检测，发现转化产物中有1个主要氧化产物3，4-dihydro-4-hydroxy-1(2H)—naphthalenone，占96．16％。另外3个氧化产物为：3，4-dihvdro-1(2H)—naphthalenone，1，2．3，4-tetrahydro-1-naphthalenol和1，2，3，4-tetrahydro-1，2-naphlhalenediol。该微生物催化氧化反应发生在四氢萘的非活性的饱和碳上，显示出海洋真菌Hypoxylon oceanicum(326#）具有独特的生物氧化机制。

Biotransformation of 1,2,3,4- Tetrahydronaphthalene by Marine Fungus Hypoxylon oceanicum

1,2,3,4- Tetrahydronaphthalene was biotransformed by marine fungus Hypoxylon oceanicum(326#)fromthe South China Sea.Glucose(10g/L),peptone(2g/L),yeastextract(1g/L),and20%arti-ficial seawater(ASW)were mixed to prepare the liquid medium for the marine fungus.A sterilized substrate,1,2,3,4-tetrahydronaphthalene(200mg/L)was distributed in the culture flasks and fermented for7days.The biotransformation products was successively extracted with EtOAc,and was measured by GC-MS and NMR.The results showed that there were four oxidative products:major product3,4-dihydro-4-hydroxy-1(2H)-naphthalenone(96.16%of the total)and three minor products:3,4-dihydro-1(2H)-naphthalenone,1,2,3,4-tetrahydro-naphthalenol and1,2,3,4-tetrahydro-1,2-naphthalenediol.It was interesting to observe thatthe oxidationreactions occurredatthe non-activatedalicyclic skeleton.This indicatedthat Hypoxylonocean-icumhad the unique bio-oxidation mechanism.