| 基于毛细管电泳-安培检测的新生儿苯丙酮尿症早期无创诊断 |
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| 引用本文： | 王伟宇,王宜萍,张美江,张军波,张东丽,楚清脆,叶建农.基于毛细管电泳-安培检测的新生儿苯丙酮尿症早期无创诊断[J].分析测试学报,2010,29(11). |
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| 作者姓名： | 王伟宇 王宜萍 张美江 张军波 张东丽 楚清脆 叶建农 |
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| 作者单位： | 华东师范大学化学系;华东师范大学体育与健康学院; |
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| 基金项目： | 国家自然科学基金资助项目，上海市科委基础研究基金资助项目 |
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| 摘 要： | 首次采用毛细管电泳-安培检测法(CE-AD)同时测定了苯丙酮尿症(Phenylketonuria,PKU)患者尿液中的两种标志物:具有电活性的苯丙酮酸及非电活性的苯乙酸。考察了运行液添加剂、SDS浓度、工作电极电位等因素对分离和检测的影响。在优化实验条件下,两种标志物在15 m in内可实现基线分离。苯丙酮酸和苯乙酸的检出限(S/N=3)分别为0.50、0.92 mg/L。苯丙酮酸、苯乙酸浓度与峰高分别在3.0～1.0×103mg/L、2.0～5.0×103mg/L范围内呈良好线性,峰高与迁移时间的相对标准偏差分别为4.4%、3.2%(苯丙酮酸)和2.2%、4.3%(苯乙酸)。该方法已成功用于PKU患儿尿液中两种标志物的测定,且完全避免了尿液中尿酸对标志物测定的干扰,有望用于PKU患儿的早期无创诊断。
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| 关 键 词： | 毛细管电泳-安培检测 苯丙酮尿症 苯丙酮酸 苯乙酸 |
Early Noninvasive Diagnosis of Phenylketonuria of Infants by Capillary Electrophoresis with Amperometric Detection |
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| WANG Wei-yu,WANG Yi-ping,ZHANG Mei-jiang,ZHANG Jun-bo,ZHANG Dong-li,CHU Qing-cui,YE Jian-nong.Early Noninvasive Diagnosis of Phenylketonuria of Infants by Capillary Electrophoresis with Amperometric Detection[J].Journal of Instrumental Analysis,2010,29(11). |
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| Authors: | WANG Wei-yu WANG Yi-ping ZHANG Mei-jiang ZHANG Jun-bo ZHANG Dong-li CHU Qing-cui YE Jian-nong |
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| Institution: | WANG Wei-yu1,WANG Yi-ping1,ZHANG Mei-jiang2,ZHANG Jun-bo1,ZHANG Dong-li1,CHU Qing-cui1,YE Jian-nong1(1.Department of Chemistry,East China Normal University,Shanghai 200062,China,2.School of Sports and Health Care,China) |
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| Abstract: | A capillary electrophoresis with amperometric detection(CE-AD) method was developed for the simultaneous determination of the marked compounds of phenylketonuria(PKU),including electroactive phenylpyruvic acid and non-elecroactive phenylacetic acid,in infant urine samples.Several important parameters such as running buffer additive,sodium dodecyl sulfate(SDS) concentration and working electrode potential were investigated.Under the optimum conditions,phenylpyruvic acid and phenylacetic acid could be well se... |
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| Keywords: | capillary electrophoresis with amperometric detection phenylketonuria(PKU) phenylpyruvic acid phenylacetic acid |
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