| 植物内源激素的反相高效液相色谱法测定 |
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| 引用本文： | 谢君, 张义正.植物内源激素的反相高效液相色谱法测定[J].分析测试学报,2001,20(1):60-62. |
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| 作者姓名： | 谢君 张义正 |
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| 作者单位： | 四川大学生命科学学院分子生物学实验室, |
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| 摘 要： | 报道了以6－N－苄基腺嘌呤（BA）为内标的反相高效液相色谱法测定玉米素（ZT）、赤霉素（GA3）、激动素（KT）、3－吲哚乙酸（IAA）和脱落酸（ABA）等5种植物内源激素的条件；采用μBondapakC18柱、乙腈－甲醇－0．6％（φ）乙酸流动相、检测波长254nm，建立了一种从植物中提取5种激素的样品处理方法，并测定了马铃薯块茎中的5种植物内源激素的含量。
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| 关 键 词： | 植物 内源激素 反相高效液相色谱 内标法 测定 |
| 文章编号： | 1004－4957(2001)01－0060－03 |
| 修稿时间： | 2000年4月6日 |
Determination of Plant Intrinsic Hormones by Reversed-phase High-performance Liquid Chromatography |
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| XIE Jun.Determination of Plant Intrinsic Hormones by Reversed-phase High-performance Liquid Chromatography[J].Journal of Instrumental Analysis,2001,20(1):60-62. |
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| Authors: | XIE Jun |
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| Abstract: | A method for the determination of 5 intrinsic hormones:zeatin(ZT), gibberellin(GA3), kinetin(KT), 3-indoleacetic acid(IAA) and abscisic acid(ABA), in plant by reversed-phase high-performance liquid chromatography with μ Bondapak C18 column and UV detector was developed. The optimal chromatographic conditions were selected. An acetonitrile－methanol－0.6 ％ acetic acid system was used as mobile phase and 6-N-benzyl adenine as an internal standard. A method for the extraction of the 5 hormones and the extract preparation was established. A sample of potato was analyzed. The mass fractions of ZT, GA3, KT, IAA and ABA are 0.298 5× 10－9, 253.2× 10－9, 2.154× 10－9, 112.5× 10－9 and 0.652 9× 10－9, respectively. The proposed method is simple, convenient and sensitive. |
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| Keywords: | Plant Intrinsic hormones Reversed _ phase high _ performance liquid chromatography Internal standard method |
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