| 用硝基苯萃取法在拉曼测试中猝灭荧光 |
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| 引用本文： | 柯惟中,余多慰.用硝基苯萃取法在拉曼测试中猝灭荧光[J].分析测试学报,1994,13(2):46-49. |
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| 作者姓名： | 柯惟中 余多慰 |
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| 作者单位： | 南京师范大学理化中心 |
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| 摘 要： | 本文报道了一种测试拉曼光谱时把硝基苯掺入蛋白质溶液再加以萃取的预处理方法，该方法能迅速猝灭蛋白质分子的荧光。得到稳定清晰的拉曼图谱，而且不影响对蛋白质分子的构象分析。
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| 关 键 词： | 蛋白 荧光 硝基苯 萃取 慢光谱 |
The Means of Treatement on Adding Nitrobenzene for Quenching Fluorescence in Raman Measurement |
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| Ke Weizhong,Yu Duowei.The Means of Treatement on Adding Nitrobenzene for Quenching Fluorescence in Raman Measurement[J].Journal of Instrumental Analysis,1994,13(2):46-49. |
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| Authors: | Ke Weizhong Yu Duowei |
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| Abstract: | The pretreatment means of extraction by adding nitrobenzene to protein aqueous solution have been reported.Through the means, the fluorescence of protein molecules has been quenched quickly. The stable, clear Raman spectra have been obtained and the conformation analysis of protein molecules has not been influened. |
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| Keywords: | Raman scattering Protein Fluorescence Nitrobenzene |
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