液相色谱-串联质谱法同时测定蔬菜中7种三唑醇类杀菌剂的残留量

采用高效液相色谱-串联质谱(HPLC-MS/MS)建立了同时测定蔬菜中环唑醇、三唑醇、粉唑醇、戊唑醇、己唑醇、烯唑醇和联苯三唑醇残留量的分析方法.样品经甲醇提取,分散固相萃取净化后,采用C8色谱柱(150 mm×2.1 mm,3 μm)分离,以甲醇-0.05%甲酸梯度洗脱,串联质谱测定,外标法定量.在优化实验条件下,7...

Simultaneous Determination of 7 Triazole Alcohol Fungicides in Vegetables by Liquid Chromatography-Tandem Mass Spectrometry

A liquid chromatography-tandem mass spectrometric method was developed for the simultaneous determination of seven triazole alcohol fungicides,including cyproconazole,triadimenol,flutriafol,tebuconazole,hexaconazole,diniconazole and bitertanol,in vegetables.Seven triazole alcohol fungicides were extracted from samples with methanol,and cleaned up by dispersive solid phase extraction.The separation of target compound was carried out on a C8 column using methanol-0.05% formic acid as mobile phase.The quantitative analysis of seven triazole alcohol fungicides were performed by tandem mass spectrometry with the external standard method.The results indicated that the calibration curves for the seven triazole alcohols were linear in the range of 1.0-20 μg/L with correlation coefficients more than 0.997,and the quantitation limits for the triazole alcohols were 10 μg/kg.The spiked recoveries for seven triazole alcohol fungicides from pea,tomato and qinggengcai matrices were in the range of 70%-100% with relative standard deviations not more than 10.7%.This method was simple and accurate,and was suitable for the simultaneous determination of seven triazole alcohol fungicides in vegetables.