离子色谱法测定奶制品及蜂王浆中的乙酰胆碱

建立了奶制品及蜂王浆中乙酰胆碱的离子交换/电导检测离子色谱分析方法.样品经去离子水超声提取,离子交换/电导检测离子色谱法测定,外标法定量.采用IonPac CS17(250 mm x4.0 mm i.d.)分析柱,流动相为甲基磺酸,梯度洗脱,流速1.0 mL/min,柱温30℃,检测池温度35℃.实验结果表明,氯化乙酰...

Determination of Acetylcholine in Dairy Product and Royal Jelly by Ion Chromatography

A ionexchange chromatographic method with conductivity detection was developed for the determination of acetylcholine(Ach) residue in dairy products and royal jelly.The sample was extracted with ultra-pure water by ultrasonic,and then determined by ion chromatography.The quantification was performed by the external standard,using IonPac CS17(250 mm×4.0 mm i.d.) column as analytical column,and methylsulfonic acid(MSA)as mobile phase at a flow rate of 1.0 mL/min.The column temperature was set at 30 ℃,and the detection cell temperature was set at 35 ℃.The calibration curve was linear between the peak area and the Ach concentration in the range of 0.02-2.0 mg/L with a correlation coefficient of 0.999 9.The limit of detection of Ach was 0.3 mg/kg in milk,yogurt and royal jelly matrices,and 1.0 mg/kg in milk powder matrix.The average recoveries of Ach from spiked dairy products and royal jelly sample ranged from 90% to 98% with RSDs of 3.0%-6.2%.