盐酸小檗碱含量测定的荧光新方法研究

研究了盐酸小檗碱(BER)在八元瓜环水溶液中的荧光光谱行为。八元瓜环与BER发生超分子相互作用后,使溶液中BER的荧光强度增加,且BER的浓度在1.90×10-8～1.00×10-5mol.L-1范围内与荧光强度呈良好的线性关系(r=0.996 1),据此建立了一种测定BER含量的荧光新方法。该方法的检出限为1.90×10-8mol.L-1,且不需对样品进行分离提纯,可直接在水溶液中简单准确地测定BER的含量,避免了有机溶剂和萃取剂的使用,具有灵敏度高、选择性好、线性范围宽等特点。该法对片剂、血清和尿样中BER含量的测定结果与药典方法较一致。

A Novel Fluorimetric Method for the Determination of Berberine Hydrochloride

The fluorescence spectral characteristic of berberine hydrochloride(BER) was studied in the cucurbit(n=8)uril(Q8]) aqueous medium.In this medium,Q8] and BER formed a supramolecular compound which resulted in the increase of BER fluorescence intensity.A good linear relationship between fluorescence intensity of the system and BER concentration was obtained.Based on this,a novel fluorimetric method was developed for the determination of BER concentration in the cucurbit(n=8)uril medium.Under the optimum conditions,the calibration curve was linear over BER concentration range of 1.90×10-8-1.00×10-5 mol·L-1,with a correlation coefficient(r) of 0.996 1 and a detection limit of 1.90×10-8 mol·L-1.The proposed method was simple,accurate,and could avoid using the toxic solvents and tedious solvent-extraction.And the method was applied in the determination of BER in tablets,serum and urine sample,and the result was in good consistence with those obtained by the pharmacopoeia method.