| MALDI-TOF-TOF鉴定IPG IEF分离的蛋白质 |
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| 引用本文： | 蒙根,李卫华,王鸿丽,谷苗,刘锋,王杰,魏开华.MALDI-TOF-TOF鉴定IPG IEF分离的蛋白质[J].分析测试学报,2004,23(Z1):74-75. |
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| 作者姓名： | 蒙根 李卫华 王鸿丽 谷苗 刘锋 王杰 魏开华 |
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| 作者单位： | 军事医学科学院,国家生物医学分析中心,北京,100850 |
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| 基金项目： | 国家863高技术发展项目(2001AA233041) |
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| 摘 要： | K562细胞是一株分化差、恶性程度高的人白血病细胞。研究表明,在一些分化诱导剂的作用下,细胞可以向红细胞系、粒细胞系和巨K562核细胞系方向分化成熟,并表现出相应血细胞类型的成熟标志。用一维固相pH梯度等电聚焦电泳(IPGIEF)分离K562细胞总蛋白质,其每一条带常包含多个蛋白质,难以用肽指纹谱技术来鉴定。有人探索过基于液相色谱的串联质谱技术来鉴定混合蛋白质,但灵敏度上存在一些问题。本文尝试采用基质辅助激光解吸附电离-串联飞行时间质谱(MALDI-TOF-TOF)分析一维电泳条带,鉴定了K562细胞中部分可能与白血病相关的蛋白质。
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Identification of IPG IEF Separated Proteins by MALDI-TOF-TOF |
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| MENG Gen,LI Wei-hua,WANG Hong-li,GU Miao,LIU Feng,WANG Jie,WEI Kai-hua.Identification of IPG IEF Separated Proteins by MALDI-TOF-TOF[J].Journal of Instrumental Analysis,2004,23(Z1):74-75. |
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| Authors: | MENG Gen LI Wei-hua WANG Hong-li GU Miao LIU Feng WANG Jie WEI Kai-hua |
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| Abstract: | Proteins from human erythroleukemia K562 were separated with IPG IEF, 27 bands were excised and then digested in gel. MALDI - TOF - TOF Ultraflex was used for protein identification under automatic MS - MS mode. 45 proteins, such as heterogeneous nuclear ribonucleoprotein, prohibitin, chaperonin, were identified with more than 3 MS - MS data for each protein. The highest score of 213 matched with 7 peptides. |
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| Keywords: | TOF-TOF Protein identification Tandem MS Proteomics Human erythroleukemia |
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