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| 吡虫啉与牛血红蛋白相互作用的光谱研究 | |
| 引用本文： | 周秋华,王彦卿,张红梅,张根成,费正皓,刘总堂.吡虫啉与牛血红蛋白相互作用的光谱研究[J].分析测试学报,2007,26(3):368-372. |
| 作者姓名： | 周秋华 王彦卿 张红梅 张根成 费正皓 刘总堂 |
| 作者单位： | 1. 盐城师范学院,江苏省滩涂生物资源与环境保护重点建设实验室,江苏,盐城,224002;盐城师范学院,应用化学与环境工程研究所,江苏,盐城,224002 2. 盐城师范学院,应用化学与环境工程研究所,江苏,盐城,224002 3. 盐城师范学院,江苏省滩涂生物资源与环境保护重点建设实验室,江苏,盐城,224002 |
| 基金项目： | 江苏省科技厅科技计划 , 江苏省滩涂生物资源与环境保护重点建设实验室基金 |
| 摘 要： | 利用紫外可见光谱和荧光光谱研究了在生理pH值条件下农药吡虫啉与牛血红蛋白(BHb)的相互作用。实验结果表明:吡虫啉分子与BHb发生反应生成复合物,导致BHb内源荧光的猝灭,该猝灭属于静态猝灭。测定了不同温度下该反应的表观结合常数、结合位点数及结合热力学参数,热力学参数的变化表明上述作用过程是一个熵增加、自由能降低的自发分子间作用过程,吡虫啉与BHb之间以静电和疏水作用力为主;并用同步荧光光谱法探讨了吡虫啉对BHb构象的影响。 |
| 关 键 词： | 吡虫啉 牛血红蛋白 紫外可见光谱 荧光光谱 热力学参数 |
| 文章编号： | 1004-4957（2007）03-0368-05 |
| 收稿时间： | 2006-05-14 |
| 修稿时间： | 2006-08-06 |
Spectroscopic Studies on the Interaction between Imidacloprid and Bovine Hemoglobin (BHb) |
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| ZHOU Qiu-hua,WANG Yan-qing,ZHANG Hong-mei,ZHANG Gen-cheng,FEI Zheng-hao,LIU Zong-tang.Spectroscopic Studies on the Interaction between Imidacloprid and Bovine Hemoglobin (BHb)[J].Journal of Instrumental Analysis,2007,26(3):368-372. | |
| Authors: | ZHOU Qiu-hua WANG Yan-qing ZHANG Hong-mei ZHANG Gen-cheng FEI Zheng-hao LIU Zong-tang |
| Institution: | 1. Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers College, Yancheng 224002, China; 2. Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers College, Yancheng 224002, China |
| Abstract: | The interaction between imidacloprid and bovine hemoglobin(BHb) was studied using UV/Vis and fluorescence spectroscopy.Experimental results showed that the quenching of BHb fluorescence by imidacloprid was a static one and was attributed to the formation of imidacloprid-BHb complex.The binding site number n,apparent binding constant KA and corresponding thermodynamic parameters were measured at different temperatures.The variations of the thermodynamic parameters indicated that the process of binding imidacloprid molecule in BHb was a spontaneous molecular interaction procedure in which the entropy increased and the Gibbs free energy decreased.The electrostatic and hydrophobic interactions played a major role in stabilizing the complex.The effect of imidacloprid on the conformation of BHb was analyzed with synchronous fluorescence spectrometry. |
| Keywords: | Imidacloprid BHb UV/Vis spectroscopy Fluorescence spectroscopy Thermodynamic parameters |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |