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铅笔芯电极表面多层自组装膜制备及其对牛血清白蛋白的测定
引用本文:武耐英,高伟,张明,刘洋,徐茂田.铅笔芯电极表面多层自组装膜制备及其对牛血清白蛋白的测定[J].分析测试学报,2012,31(6):691-695.
作者姓名:武耐英  高伟  张明  刘洋  徐茂田
作者单位:1. 商丘师范学院 化学化工学院 纳米生物分析化学河南省高校重点实验室培育基地,河南 商丘,476000
2. 晨光生物科技集团股份有限公司,河北 曲周,057250
基金项目:国家自然科学基金项目(21105062,21175091);河南省科技攻关项目(102102310392);商丘师范学院青年基金项目(2010QN17)
摘    要:采用电沉积技术将金沉积在铅笔芯电极(PGE)上,借助Au-S作用,将L-半胱氨酸(L-Cys)组装于金表面,利用吖啶橙(AO)与L-半胱氨酸之间的静电作用,将吖啶橙间接组装于金表面,构建成三层自组装膜电极AO/L-Cys/Au/PGE。将该复合膜电极连接在电化学工作站的工作电极和辅助电极端之间,与参比电极浸入溶液中,构建了一个新的检测回路。采用电化学阻抗谱技术和循环伏安法对多层自组装膜的组装过程进行表征。利用吖啶橙与牛血清白蛋白(BSA)间的相互作用,采用零流电位法,通过对比加入不同浓度BSA后零流电位值的变化(ΔEzcp)对BSA进行检测。结果显示,在1.0×10-9~1.0×10-5mol/L范围内,ΔEzcp与BSA浓度的对数值呈良好线性,相关系数(r)为0.997 0,检出限为1.41×10-11mol/L。该方法选择性和重复性好,有望应用于其它蛋白的测定。

关 键 词:零流电位法  牛血清白蛋白  铅笔芯电极  多层自组装膜

Preparation of Self-assembled Multilayer Membrane on Pencil Graphite Electrode and Its Determination for Bovine Serum Albumin
WU Nai-ying , GAO Wei , ZHANG Ming , LIU Yang , XU Mao-tian.Preparation of Self-assembled Multilayer Membrane on Pencil Graphite Electrode and Its Determination for Bovine Serum Albumin[J].Journal of Instrumental Analysis,2012,31(6):691-695.
Authors:WU Nai-ying  GAO Wei  ZHANG Ming  LIU Yang  XU Mao-tian
Institution:1(1.Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry,College of Chemistry and Chemical Engineering,Shangqiu Normal University,Shangqiu 476000,China;2.Chenguang Biotech Group Co.,Ltd.,Quzhou 057250,China)
Abstract:An electro-deposition method was adopted to prepare gold-capped pencil graphite surface using pencil graphite electrode(PGE).By chemical bonding of Au-S,L-cysteine(L-Cys) can be stably immobilized onto the surface of gold,followed by an assembling with acridine orange(AO) via the electrostatic interaction between AO and L-cysteine to obtain an AO/L-Cys/Au/PGE multilayer membrane.The prepared multilayer membrane was connected between the terminal joints of the working electrode and the counter electrode,together with a reference electrode,to construct a novel system.The whole multilayer membrane fabrication process was characterized by cyclic voltammetry and electrochemical impedance spectroscopy.The interaction of AO and the subsequent introduction of bovine serum albumin(BSA) resulted in a significant change of zero current potential(ΔEzcp) utilizing zero current potentiometry,thus the electrochemical quantification of BSA could thus be realized.The results showed that ΔEzcp and logarithmic of BSA concentration was linear in a wide concentration range of 1.0×10-9-1.0×10-5 mol/L(r=0.997 0),and the detection limit was 1.41×10-11 mol/L.The multilayer membrane exhibited good selectivety and reproducibility,and was applicable for the determiantion of other proteins.
Keywords:zero current potentiometry  bovine serum albumin  pencil graphite electrode  self-assembled multilayer membrane
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