| MALDI-TOF-MS测定溶菌酶的相对分子质量中在线纯化技术的应用 |
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| 引用本文： | 孙淑清,杜萍,陈代梅,季怡萍,刘淑莹.MALDI-TOF-MS测定溶菌酶的相对分子质量中在线纯化技术的应用[J].分析测试学报,2004,23(2):87-88,91. |
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| 作者姓名： | 孙淑清 杜萍 陈代梅 季怡萍 刘淑莹 |
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| 作者单位： | 天津大学,理学院,化学系,天津,300072;中国科学院长春应用化学研究所,吉林,长春,130021 |
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| 摘 要： | 用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)测定溶菌酶的相对分子质量，采用硝酸纤维素膜作为溶菌酶的固相载体，在质谱仪上进行在线纯化溶菌酶样品，在测试前尽可能除去样品中的添加剂，以消除一些盐和蛋白质变性剂抑制样品峰的情形；此法简单、快速，并可明显增强离子峰的强度，提高测试的灵敏度。
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| 关 键 词： | 基质辅助激光解吸电离飞行时间质谱 溶菌酶 在线纯化 相对分子质量 |
| 文章编号： | 1004-4957(2004)02-0087-02 |
An Online Purification Technique for Relative Molecular-mass Determination of Lysozyme by MALDI-TOF-MS |
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| SUN Shu-qing,DU Ping,CHEN Dai-mei,JI Yi-ping,LIU Shu-ying.An Online Purification Technique for Relative Molecular-mass Determination of Lysozyme by MALDI-TOF-MS[J].Journal of Instrumental Analysis,2004,23(2):87-88,91. |
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| Authors: | SUN Shu-qing DU Ping CHEN Dai-mei JI Yi-ping LIU Shu-ying |
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| Institution: | SUN Shu-qing1,DU Ping1,CHEN Dai-mei1,JI Yi-ping2,LIU Shu-ying2 |
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| Abstract: | A new method for sample purification is described for the determination of relative molecular mass of target proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS). In order to remove all kinds of additives in the lysozyme sample such as salts, surfactants and denaturants that are likely to suppress the signal intensity, the sample was purified on line with a nitrocellulose membrane as the solid-phase carrier prior to the MALDI-TOF-MS test. The results indicate that this new method is simple, rapid and more sensitive, as the signal intensity is enhanced. |
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| Keywords: | Matrix assisted laser desorption/ionization time-of-flight mass spectrometry Lysozyme Online purification Relative molecular mass |
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