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| 基于分子模拟的脱氧雪腐镰刀菌烯醇半抗原分子设计及免疫效果研究 | |
| 引用本文： | 孙秀兰,金萍,张银志.基于分子模拟的脱氧雪腐镰刀菌烯醇半抗原分子设计及免疫效果研究[J].分析测试学报,2010,29(7). |
| 作者姓名： | 孙秀兰 金萍 张银志 |
| 作者单位： | 1. 江南大学,食品学院,江苏,无锡,214122;江南大学,食品科学与技术国家重点实验室,江苏,无锡,214122 2. 江南大学,食品学院,江苏,无锡,214122 3. 江南大学,食品科学与技术国家重点实验室,江苏,无锡,214122 |
| 基金项目： | 国家863高技术研究发展计划资助项目，国家自然科学基金资助项目，"十一五"国家科技支撑计划资助项目 |
| 摘 要： | 运用分子模拟软件Hyperchem 7.5,对脱氧雪腐镰刀菌烯醇(DON)及其6种不同连接臂修饰后的DON衍生物的分子构型进行量化计算,通过对其结构参数以及分子轨道的计算比较,获得高特异性、高生物活性的DON半抗原结构,避免了半抗原设计方面的盲目性。同时合成了其中的3种半抗原,通过碳二亚胺(EDC)法与BSA进行偶联免疫Bal b/c小鼠,制备多抗血清,采用间接酶联免疫吸附法(ELISA)和间接竞争ELISA法进行抗体效果的比较。结果表明,分子模拟计算结果与免疫实验的结果相一致,分子模拟对半抗原结构设计具有指导性意义。 |
| 关 键 词： | 分子模拟 奥斯汀模型1(AM1) 脱氧雪腐镰刀菌烯醇(DON) 连接臂 酶联免疫检测方法(ELISA) |
Hapten Design of Deoxynivalenol Using Molecular Simulation and Its Immune Effects |
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| SUN Xiu-lan,JIN Ping,ZHANG Yin-zhi.Hapten Design of Deoxynivalenol Using Molecular Simulation and Its Immune Effects[J].Journal of Instrumental Analysis,2010,29(7). | |
| Authors: | SUN Xiu-lan JIN Ping ZHANG Yin-zhi |
| Institution: | SUN Xiu-lan1,2,JIN Ping1,ZHANG Yin-zhi2(1.School of Food Science & Technology,Jiangnan University,Wuxi 214122,China,2.The State Key Lab of Food Science and Technology,China) |
| Abstract: | Molecular modeling with software Hyperchem 7.5 was used to calculate quantitatively the molecular structures of deoxynivalenol(DON) and its derivatives obtained by modifying DON with six different link arms.Based on the calculation of their structural parameters and molecular orbitals,high specificity and high biological activity of DON hapten structure were obtained.Three of the haptens were then conjugated to BSA using the carbodiimide(EDC) method.Polyclonal antibodies to DON were prepared from Bal b/c mi... |
| Keywords: | Hyperchem 7 5 |
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