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高效液相色谱法同时测定香兰素与邻位香兰素
引用本文:金海涛,马健瑜,王晓珣,陈星,任丽艳,关瑾,李云.高效液相色谱法同时测定香兰素与邻位香兰素[J].分析测试学报,2011,30(2):222-224.
作者姓名:金海涛  马健瑜  王晓珣  陈星  任丽艳  关瑾  李云
作者单位:沈阳化工大学应用化学学院;
基金项目:辽宁省自然基金资助项目(20090655); 辽宁省教育厅2008年度科研计划项目(2008S184)
摘    要:建立了同时测定香兰素和邻位香兰素的高效液相色谱法.考察了流动相组成、柱温等因素对分析效率的影响.在流动相为5% 乙酸-乙腈(60 :40,体积比),流速1.0 mL/min,柱温25 ℃的优化条件下,香兰素和邻位香兰素可在5 min内实现分离.测定结果表明,香兰素和邻位香兰素在10 ~240 mg/L范围内线性关系良好...

关 键 词:高效液相色谱法  香兰素  邻位香兰素  同分异构体

Simultaneous Determination of Vanillin and o Vanillin by High Performance Liquid Chromatography
JIN Hai tao,MA Jian yu,WANG Xiao xun,CHEN Xing,REN Li yan,GUAN Jin,LI Yun.Simultaneous Determination of Vanillin and o Vanillin by High Performance Liquid Chromatography[J].Journal of Instrumental Analysis,2011,30(2):222-224.
Authors:JIN Hai tao  MA Jian yu  WANG Xiao xun  CHEN Xing  REN Li yan  GUAN Jin  LI Yun
Institution:JIN Hai-tao,MA Jian-yu,WANG Xiao-xun,CHEN Xing,REN Li-yan,GUAN Jin,LI Yun(Institute of Applied Chemistry,Shenyang University of Chemical Technology,Shenyang 110142,China)
Abstract:A high performance liquid chromatographic method was developed for the simultaneous separation and determination of vanillin and o-vanillin.Influences of mobile phase composition and column temperature on the separation were investigated.Under the condition of 5% acetic acid-acetonitrile(60∶40,by volume) as mobile phase at a flow rate of 1.0 mL/min and 25 ℃,the vanillin and o-vanillin were separated in less than 5 min.The calibration curves of vanillin and o-vanillin showed good linearities in the range of 10-240 mg/L with both correlation coefficients of 0.999 7.The limits of detection(S/N=3) for vanillin and o-vanillin were 0.06 mg/L and 0.02 mg/L,respectively.The average recoveries at three spiked levels were in the range of 99%-102% with relative standard deviations of 0.2%-0.6%.The method was successfully used for the determination of vanillin and o-vanillin in real samples with satisfactory results.
Keywords:high performance liquid chromatography  vanillin  o-vanillin  isomers  
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