基于纳米粒子的光学细胞传感器在癌细胞研究中的应用

作为一种极为灵敏、快速的新型生物检测技术,光学细胞传感器在生物医学领域的研究应用备受关注,成为当今生物分析化学领域的研究前沿和热点.它是以细胞作为传感元件来研究信号识别、传导和指示的过程,在毒性物质、病原体等外界条件作用下,研究细胞中活性分子及其生理条件的变化,通过光学信号的变化定量分析细胞膜表面分子、胞内酶分子及微环...

Application of Nanoparticles-based Optical Cell Biosensor in the Research of Cancer Cells

Optical biosensors based on different optical phenomena have been used widely over the past decade to analyse biomolecular interactions.With the advent of the nanoparticle technology,the probing of individual living cells,and the measuring of chemical species in specific locations within the cell for living cell sensing have become possible.Optical biosensors based on nanoparticles offer high sensitivity,specificity and rapid detection in the study of cancer cell or biomarker.In this paper,the applications of nanoparticles-based optical biosensor for the specific identification of molecules on the cell membrane surface for the study of cancer cells,the research of enzyme molecule in cancer cells and intracellular and the reactive oxygen,glutathione and physiological pH value in the smallest environment are reviewed.The prospect of this novel technique is also discussed.