| 二维高效液相色谱技术用于分析中药制剂中麻黄类生物碱 |
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| 引用本文： | 邹萍,王绍艳,张志强,吴秀红.二维高效液相色谱技术用于分析中药制剂中麻黄类生物碱[J].分析测试学报,2007,26(1):120-121,124. |
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| 作者姓名： | 邹萍 王绍艳 张志强 吴秀红 |
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| 作者单位： | 辽宁科技大学,化工学院,辽宁,鞍山,114044 |
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| 基金项目： | 辽宁省教育厅高等学校科学研究项目资助(202193400) |
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| 摘 要： | 构建了一个离线的二维高效液相色谱系统,以解决成分复杂的中药样品的分离和痕量组分的定量问题。该系统在原有的单泵、单检测器高效液相色谱仪的基础上作了相应的改进而构成,样品先在第一维的Zor-bax SB-C18色谱柱中进行分离之后,利用一个六通切换阀将目标组分切换并收集,浓缩后再注入第二维的Ai-chrom Bond-AQ C18色谱柱中进行分离。利用上述方法对3种麻黄类生物碱进行了二维分离和分析,实验结果表明,样品的分离明显改善,并且这个系统可用于中药的直接进样分析。
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| 关 键 词： | 二维高效液相色谱 离线 麻黄类生物碱 |
| 文章编号： | 1004-4957（2007）01-0120-02 |
| 收稿时间： | 2006-04-05 |
| 修稿时间： | 2006-04-052006-05-03 |
Analysis of Ephedrine Alkaloids in Chinese Medicine by 2D HPLC |
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| ZOU Ping,WANG Shao-yan,ZHANG Zhi-qiang,WU Xiu-hong.Analysis of Ephedrine Alkaloids in Chinese Medicine by 2D HPLC[J].Journal of Instrumental Analysis,2007,26(1):120-121,124. |
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| Authors: | ZOU Ping WANG Shao-yan ZHANG Zhi-qiang WU Xiu-hong |
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| Institution: | School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114044, China |
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| Abstract: | An off-line 2D HPLC was set up in the laboratory for separating and determining complex components in traditional Chinese medicines.It is based on a conventional HPLC system with one-pump and onedetector.Column-switching technique was applied to the system.The sample was first separated by passing through the column(Zorbax SB-C18) of the first dimension of 2D HPLC.After eluting,the fraction of interest was collected and concentrated.The concentrate was then injected into the second column(Aichrom Bond-AQ C18) of the second dimension of 2D HPLC.This system has been used to separate and determine the ephedrine alkaloids.Experimental results showed that the separation of the alkaloids was substantially improved. |
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| Keywords: | Multidimensional high performance liquid chromatography Off-line Ephedrine alkaloids |
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