人血清白蛋白与氨基酸对映异构体的差异性识别研究

以人血清白蛋白为识别主体,利用表面等离子体共振(SPR)技术实时研究了其与5种氨基酸对映异构体相互作用过程中的动态行为。实验结果显示,每种氨基酸分子的L-和D-型异构体在与人血清白蛋白作用过程中均存在明显的动力学差异。动力学数据分析表明,虽然各种氨基酸的L-和D-型异构体与人血清白蛋白作用的结合速率常数、解离速率常数无统一规律,但在解离平衡常数或结合平衡常数上有一致性。每种氨基酸的L-型异构体与人血清白蛋白的亲和力均大于D-型,这表明人血清白蛋白在与氨基酸对映异构体作用的过程中基于手性识别作用而产生了明显的差异性结合。

Chiral Recognition of Interactions between HSA and Amino Acid Enantiomers

The interactions between human serum albumin(HSA)and five kinds of amino acid enantiomers were studied by surface plasmon resonance (SPR) without any labeling for the first time.The dynamic informations of their interactions were well shown in real time,and the kinetics parameters were also obtained.The difference in interactions between each of amino acid enantiomers and HSA could be recognized obviously by comparison of the sensorgrams.Although the binding rate constants and the dissociation rate constants of them did not have any rule,the results showed that the affinity of HSA and L-amino acid was bigger than that of HSA and D-amino acid,which indicated the chiral recognition force may contribute to the discrimination interactions between amino acid enantiomers and HSA.