表面等离子体共振技术在蛋白质-蛋白质相互作用研究中的应用

表面等离子共振(SPR)近年来迅速发展为用于分析生物分子相互作用的一项技术.该技术无需标记、特异性强、灵敏度高、样品用量小,可实现在线连续实时检测.目前SPR已被广泛应用于免疫学、蛋白质组学、药物筛选、细胞信号转导、受体/配体垂钓等领域.该文阐述了基于表面等离子体共振技术生物传感器的基本原理和技术流程,综述了SPR在蛋白质-蛋白质相互作用动力学研究、蛋白质结构及功能研究、蛋白质突变和碎片分析、信号转导中的应用以及SPR在蛋白质-蛋白质相互作用研究中的多项关键技术.指出SPR通过与光谱、电化学等多技术联用后,可以获得更加详实的信息.

Investigation of Protein-Protein Interactions by Surface Plasmon Resonance

The technique of surface plasmon resonance(SPR) has been rapidly developed to investigate the interactions of biomolecules in recent years due to its exceptional capabilities with respects to label-free, specificity, sensitivity, sample dosage, real-time and online detection. Recently, the applications of SPR have been extended to immunology, proteomic, drug screening, cellular signal transduction, ligand/receptor fishing and so on. In this paper, the principle and the experimental design of biosensor chip technology of SPR biosensors was firstly briefly described. Its application on protein - protein interaction, including dynamic study by measuring the equilibrium binding constants , the association - dissociation rates, structure - function study, mutation and fragment analysis, and signal transduction were reviewed. In addition, several key techniques of SPR technology used in protein - protein interaction study, including surface modification of the sensor chip, methods to resist non-specific adsorption and surface regeneration, were discussed. When coupled with other spectral, electrochemical techniques, SPR biosensors can extend the capability to obtain more specific interfacing properties during the protein - protein interaction process.