pH‐responsive destabilization and facile bioconjugation of new hydroxyl‐terminated block copolymer micelles |
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Authors: | Behnoush Khorsand Jung Kwon Oh |
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Institution: | Department of Chemistry and Biochemistry, Concordia University, Montreal, Quebec, Canada H4B 1R6 |
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Abstract: | New hydroxyl‐terminated amphiphilic block copolymers (HO‐ABPs) having pendant t‐butyl groups for pH‐responsiveness and terminal OH groups for bioconjugation are reported. These HO‐ABPs consist of hydrophilic poly(oligo(ethylene oxide) monomethyl ether methacrylate) and hydrophobic poly(t‐butyl methacrylate) blocks and were synthesized by a consecutive atom transfer radical polymerization in the presence of an OH‐terminated bromine initiator. Aqueous self‐assembly of HO‐ABPs resulted in colloidally stable micellar aggregates being capable of encapsulating hydrophobic guest molecules. They were nontoxic to cells and destabilized in response to low pH. A facile bioconjugation of HO‐ABP micelles for active targeting is demonstrated by conjugation with biotin (vitamin H) and competitive assay exhibiting >93% ABP chains conjugated with biotin in each micelle. © 2013 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2013 |
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Keywords: | amphiphilic block copolymer atom transfer radical polymerization (ATRP) bioconjugation controlled release diblock copolymers drug delivery systems micelles pH‐responsive degradation POEMA PtBMA |
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