New asymmetric monomethine cyanine dyes for nucleic-acid labelling: absorption and fluorescence spectral characteristics |
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| Institution: | 1. Institute of Organic Chemistry with Centre for Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;2. Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;3. Department of Chemical Technology, Faculty of Chemistry, University of Sofia, 1126 Sofia, Bulgaria;4. Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;1. Instituto de Física, Universidade do Estado do Rio de Janeiro, RJ 20559-900, Brazil;2. Departamento de Física, Pontifícia Universidade Católica do Rio de Janeiro, RJ 22453-900, Brazil;1. Faculty of Chemistry, Lomonosov Moscow State University, Moscow, Russia;2. Pohang Accelerator Laboratory, POSTECH, Pohang 37673, Republic of Korea;3. Department of Physics Education, Chungbuk National University, Cheongju 28644, Republic of Korea;4. Donetsk Physico-Technical Institute, Donetsk, Ukraine;5. Faculty of Materials Science, Lomonosov Moscow State University, Moscow, Russia;6. Institute of General and Inorganic Chemistry, Moscow, Russia;1. National Research Tomsk Polytechnic University, 30 Lenin Avenue, Tomsk 634034, Russian Federation;2. Institute for Scintillation Materials, 60 Lenin Avenue, Kharkov 61001, Ukraine;1. Department of Pharmacy & Pharmacology, Antoni van Leeuwenhoek/The Netherlands Cancer Institute and MC Slotervaart, Amsterdam, The Netherlands;2. Pharma Mar, S.A. Colmenar Viejo, Madrid, Spain;3. Division of Clinical Pharmacology, Department of Medical Oncology, The Netherlands Cancer Institute, Amsterdam, The Netherlands;4. Division of Pharmacoepidemiology and Clinical Pharmacology, Faculty of Science, Department of Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands;1. Department of General Physics, Eastern European National University, Lutsk, Ukraine;2. Faculty of Electrical Engineering, Czestochowa University of Technology, Armii Krajowej 17, Czestochowa, Poland;3. Volyn National University, Voli Ave 13, 43025 Lutsk, Ukraine;4. Department of Inorganic and Physical Chemistry, Eastern European National University, Lutsk, Ukraine;5. Wireless and Photonic Networks Research Centre, Faculty of Engineering, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;6. Lutsk National Technical University, Lutsk, Ukraine;7. Department of Inorganic and Organic Chemistry, Lviv National University of Veterinary Medicine and Biotechnologies, 50 Pekarska Street, 79010 Lviv, Ukraine;8. Institute of Physics, J. Dlugosz University Częstochowa, Armii Krajowej 13/15, Częstochowa PL-42-217, Poland |
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| Abstract: | Absorption spectra and fluorescence properties of a series of newly synthesized asymmetric monomethine cyanine dyes are studied. The dyes carry one or two positive charges. They are devoid of their own fluorescence in solution and become fluorescent upon binding to nucleic acids only. The fluorescence maxima of the new dyes are localized between 530 and 650 nm. The wavelength and intensity of fluorescence are dependent on molecular structure of the dye, type of nucleic acid and the concentration of both nucleic acid and salts. Some of the dyes are capable of distinguishing between single-stranded and double-stranded (ds) polynucleotides giving fluorescence maxima localized at different wavelengths. Detection threshold for dsDNA for most of the dyes is comparable to that of ethidium bromide. The sensitivity of the dye-dsDNA complexes to NaCl concentrations show that the new dyes interact with dsDNA by both intercalation and electrostatically. |
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