新型单羟基氯代咔咯的合成及其光断裂DNA性质

以五氟苯甲醛和吡咯经缩合反应合成了五氟苯基二吡咯烷烃(1)； 1与3-氯-4-羟基苯甲醛经缩合反应合成了一个新的10-位苯环被氯原子取代的单羟基自由咔咯--10-(3-氯-4-羟苯基)-5,15-二(五氟苯基)咔咯(〖STHZ〗2〖STBZ〗)，收率12.0%，其结构经UV-Vis， ¹H NMR， ¹⁹F NMR, HR-ESI-MS和X-射线单晶衍射表征。琼脂糖凝胶电泳实验发现：2在光照下能够有效引起超螺旋质粒pBR 322 DNA发生断裂，当浓度为100 μmol·L^-1时，活性最强，断裂百分比为68%。

Synthesis of A Novel Mono-hydroxyl Chloro-corrole and Its Photocleavage of DNA

5-(Pentafluoro-phenyl)-dipyrromethane(1) was prepared by condensation reaction of pen-tafluorobenzaldehyde with pyrrole.A novel mono-hydroxyl corrole, 10-(3-chloro-4-hydroxylphenyl)-5, 15-bis(pentafluorophenyl) corrole(2) with yield of 12.0%, was synthesized by condensation reaction of 1 with 3-chloro-4-hydroxylbenzaldehyde.The structure was characterized by UV-Vis, 1 H NMR, 19 F NMR, HR-ESI-MS and X-ray single crystal diffraction.Agarose gel electrophoresis showed that 2 could cleave 68%supercoiled DNA( form I) to give nicked-circular DNA( form II) under irradiation at 100 μmol· L-1 .