| 两相体系中羟化酶催化-1,2,3,4-四氢喹啉不对称羟基化反应 |
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| 引用本文： | 李珂,杨敏,崔宝东,陈永正.两相体系中羟化酶催化-1,2,3,4-四氢喹啉不对称羟基化反应[J].合成化学,2016,24(4):350-354. |
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| 作者姓名： | 李珂 杨敏 崔宝东 陈永正 |
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| 作者单位： | 遵义医学院 药学院，贵州 遵义 563000 |
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| 基金项目： | 国家自然科学基金资助项目(21162047)，贵州省科技厅项目(QKHGZ-2011-7017) |
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| 摘 要： | 以菌株Pseudomonas plecoglossicidas ZMU-T02整细胞为生物催化剂，1,2,3,4-四氢喹啉经不对称羟基化反应合成了(R)-4-羟基-1,2,3,4-四氢喹啉，其结构经1H NMR, 13C NMR, HR-MS和HPLC确证。在细胞浓度为30 g cdw·L-1， pH为9.0，整细胞悬浮液/有机溶剂比例为1 ：1的两相体系中，底物浓度为10 mmol·L-1时，收率37%， ee值98%。
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| 关 键 词： | 1 2 3 4-四氢喹啉 不对称羟基化 (R)-4-羟基-1 2 3 4-四氢喹啉 生物催化 合成 两相反应 |
| 收稿时间： | 2016-01-06 |
Asymmetric Hydroxylation of 1,2,3,4-Tetrahydroquinoline by Hydroxylase-catalyzed Hydroxylation in Two-phase Reaction System |
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| LI Ke,YANG Min,CUI Bao-dong,CHEN Yong-zheng.Asymmetric Hydroxylation of 1,2,3,4-Tetrahydroquinoline by Hydroxylase-catalyzed Hydroxylation in Two-phase Reaction System[J].Chinese Journal of Synthetic Chemistry,2016,24(4):350-354. |
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| Authors: | LI Ke YANG Min CUI Bao-dong CHEN Yong-zheng |
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| Institution: | School of Pharmacy, Zunyi Medical University, Zunyi 563000, China |
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| Abstract: | (R)-4-hydroxy-1,2,3,4-tetrahydroquinoline was synthesized by hydroxylation of 1,2,3,4-tetrahydroquinoline using the whole-cell of Pseudomonas plecoglossicidas ZMU-T02 as biocatalyst.The structure was confirmed by 1 H NMR, 13 C NMR, HR-MS and HPLC.Under the optimized reaction conditionscell density 30 g cdw· L-1, pH 9.0, 50%(V/V) hexadecane as co-solvent], when sub-strate concentration was 10 mmol· L-1 , the yield and ee were 37%and 98%, respectively. |
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| Keywords: | 1 2 3 4-tetrahydroquinoline asymmetric hydroxylation ( R)-4-hydroxy-1 2 3 4-tetra-hydroquinoline biocatalysis synthesis two-phase reaction |
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