Commercialized rapid immunoanalytical tests for determination of allergenic food proteins: an overview |
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Authors: | Patricia Schubert-Ullrich Judith Rudolf Parisa Ansari Brigitte Galler Manuela Führer Alexandra Molinelli Sabine Baumgartner |
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Institution: | 1.Dept. IFA-Tulln, Center for Analytical Chemistry, Christian Doppler Pilot Laboratory for Rapid Test Systems for Allergenic Food Contaminants,University of Natural Resources and Applied Life Sciences Vienna,Tulln,Austria |
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Abstract: | Food allergies have become an important health issue especially in industrialized countries. Undeclared allergenic ingredients
or the presence of “hidden” allergens because of contamination during the food production process pose great health risks
to sensitised individuals. The EU directive for food labelling lists allergenic foods that have to be declared on food products
by the manufacturers. The list includes gluten-containing cereals, crustaceans, eggs, fish, peanuts, soybeans, milk, various
nuts (e.g. almond, hazelnut, and walnut, etc.), celery, mustard, sesame seeds, lupin, and molluscs. Reliable methods for detection
and quantification of food allergens are needed that can be applied in a fast and easy-to-use manner, are portable, and need
only limited technical equipment. This review focuses on the latest developments in food allergen analysis with special emphasis
on fast immunoanalytical methods such as rapid enzyme-linked immunosorbent assays (ELISA), lateral-flow immunochromatographic
assays (LFA) and dipstick tests. Emerging technologies such as immunochemical microarrays and biosensors are also discussed
and their application to food allergen analysis is reviewed. Finally, a comprehensive overview of rapid immunochemical test
kits that are currently available commercially is given in tabular form. |
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Keywords: | Immunoassay Strip tests Microarray Biosensors Surface plasmon resonance Resonance-enhanced absorption |
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