Modelling of the impedimetric responses of an aflatoxin B1 immunosensor prepared on an electrosynthetic polyaniline platform |
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Authors: | Joseph H O Owino Anna Ignaszak Amir Al-Ahmed Priscilla G L Baker Hailemichael Alemu Jane Catherine Ngila Emmanuel I Iwuoha |
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Institution: | (1) Sensor Research Laboratory, Department of Chemistry, University of the Western Cape, Private Bag X17, Bellville, 7535 Cape Town, South Africa;(2) Chemistry Department, National University of Lesotho, P.O. Roma 180, Lesotho, South Africa;(3) School of Chemistry, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Westville, Durban, 4000, South Africa |
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Abstract: | Aflatoxins are a group of mycotoxins that have deleterious effects on humans and are produced during fungal infection of plants
or plant products. An electrochemical immunosensor for the determination of aflatoxin B1 (AFB1) was developed with AFB1antibody (AFB1-Ab) immobilized on Pt electrodes modified with polyaniline (PANi) and polystyrene sulphonic acid (PSSA). Impedimetric analysis
shows that the electron transfer resistances of the Pt/PANi–PSSA electrode, the Pt/PANi–PSSA/AFB1-Ab immunosensor and Pt/PANi–PSSA/AFB1-Ab incubated in bovine serum albumin (BSA) were 0.458, 720 and 1,066 kΩ, respectively. These results indicate that electrochemical
impedance spectroscopy (EIS) is a suitable method for monitoring the change in electron transfer resistance associated with
the immobilization of the antibody. Modelling of EIS data gave equivalent circuits which showed that the electron transfer
resistance increased from 0.458 kΩ for the Pt/PANi–PSSA electrode to 1,066 kΩ for the Pt/PANi–PSSA/AFB1-Ab immunosensor, indicating that immobilization of the antibody and incubation in BSA introduced an electron transfer barrier.
The AFB1 immunosensor had a detection limit of 0.1 mg/L and a sensitivity of 869.6 kΩ L/mg. |
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Keywords: | Aflatoxin B1 Aflatoxin B1 antibody Electrochemical impedance spectroscopy Differential pulse voltammetry Immunosensor |
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