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A novel and rapid HPGPC-based strategy for quality control of saccharide-dominant herbal materials: Dendrobium officinale,a case study |
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| Authors: | Jun Xu Song-Lin Li Rui-Qi Yue Chun-Hay Ko Jiang-Miao Hu Jing Liu Hing-Man Ho Tao Yi Zhong-Zhen Zhao Jun Zhou Ping-Chung Leung Hu-Biao Chen Quan-Bin Han |
| Institution: | 1. School of Chinese Medicine, Hong Kong Baptist University, 7 Baptist University Road, Kowloon Tong, Hong Kong SAR, China 2. Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Branch of China Academy of Chinese Medical Sciences and Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, China 3. State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N. T., Hong Kong SAR, China 4. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China |
| Abstract: | Qualitative and quantitative characterization of natural saccharides, especially polysaccharides, in herb materials remains a challenge due to their complicated structures and high macromolecular masses. Currently available methods involve time-consuming and complicated operations, and present poor specificity. Here, a novel and rapid high-performance gel permeation chromatography (HPGPC)-based approach is described for quality assessment of saccharide-dominant herbal materials by simultaneous qualitative and quantitative analysis of saccharide components. Dendrobium officinale, one of the rarest tonic herbs worldwide, was employed as the model herb in this study. First, a HPGPC fingerprint based on the molecular weight distribution of its carbohydrate components was established for qualitative identification of D. officinale. Then, HPGPC-guided dominant holistic polysaccharide marker was separated using ultra-filtration followed by HPGPC determination for quantitative evaluation of D. officinale. The experimental results suggest that this method is more efficient, stable, and convenient compared with the currently available methods for authentication and quality evaluation of D. officinale, and we expect the method will have similar advantages when used for quality control of other saccharide-dominant herbal materials and products. Graphical Abstract The characteristic HPGPC fingerprint of Dendrobium officinale compared with other confused Dendrobium species |
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