Development of fast screening methods for the analysis of veterinary drug residues in milk by liquid chromatography-triple quadrupole mass spectrometry |
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Authors: | José Luis Martínez Vidal Antonia Garrido Frenich María M Aguilera-Luiz Roberto Romero-González |
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Institution: | 1. Group “Analytical Chemistry of Contaminants”, Department of Analytical Chemistry, Almería University, 04071, Almería, Spain 2. Department of Analytical Chemistry, Almería University, 04071, Almería, Spain
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Abstract: | Two rapid multi-residue screening methods for the determination of 21 veterinary drugs in milk by ultra-high-performance liquid
chromatography-tandem mass spectrometry (UHPLC-MS/MS) have been developed and compared. For both methods, veterinary drugs
were extracted from milk samples using a rapid extraction procedure based on the modification of the well-known QuEChERS (Quick,
Easy, Cheap, Effective, Rugged, and Safe) method, and no further clean-up steps were necessary. One screening method was based
on the selection of a characteristic neutral loss or product ion of the various families of compounds, whereas another one
was based on the choice of a selected reaction monitoring (SRM) for each compound. These methods were compared with regards
to false negatives, cut-off values and the unreliability region. The total run time for both methods was 3 min, allowing quick
selection of samples that contained veterinary drugs. Non-negative samples were re-analyzed by the UHPLC-MS/MS confirmation/quantification
method, which consisted in the monitoring of two SRM for each compound. The methods were validated according to international
guides. The proposed analytical methods allow for the identification and confirmation of the target veterinary drugs at trace
levels employing quick analysis time. |
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