Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review |
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Authors: | Joana?Sousa Gilberto?Alves Ana?Fortuna Email author" target="_blank">Amílcar?Falc?oEmail author |
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Institution: | 1.Pharmacology Department, Faculty of Pharmacy,University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba,Coimbra,Portugal;2.CNC – Centre for Neurosciences and Cell Biology,University of Coimbra,Coimbra,Portugal;3.CICS-UBI – Health Sciences Research Centre,University of Beira Interior,Covilh?,Portugal |
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Abstract: | Fluoroquinolones are one of the most promising and intensively studied drugs of contemporary anti-infective chemotherapy.
New fluoroquinolone antibacterials with improved pharmacokinetic properties and a broad spectrum of activity have been developed,
opening new windows of opportunity for clinical use. To our knowledge, no comprehensive and critical review of the analytical
methods for the determination of these agents, which correspond to the third- and fourth-generation quinolones, has yet been
published. This work summarizes for the first time most of the liquid chromatographic methods reported in the literature for
the separation and quantification of the new fluoroquinolones in biological matrices and pharmaceutical formulations. A systematic
and detailed survey of physicochemical properties, sample preparation procedures, and chromatographic and detection conditions
is presented herein. In the course of this review several liquid chromatographic methods are discussed: reversed-phase high-performance
liquid chromatography (RP-HPLC), ion-exchange high-performance liquid chromatography (IEX-HPLC), hydrophilic interaction liquid
chromatography (HILIC), high-performance thin-layer chromatography (HPTLC) and other chiral chromatographic methods. Their
advantages, applicability and limitations are also examined. |
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