Amperometric biosensor for the determination of creatine |
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| Authors: | A Ramanavicius |
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| Institution: | (1) Centre of Nanotechnology and Material Science, Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 2006 Vilnius, Lithuania |
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| Abstract: | An amperometric biosensor for the determination of creatine was developed. The carbon rod electrode surface was coated with
sarcosine oxidase (SOX) and creatine amidinohydrolase by cross-linking under glutaraldehyde vapour. The SOX from Arthrobacter sp. 1–1 N was purified and previously used for creation of a creatine biosensor. The natural SOX electron acceptor, oxygen,
was replaced by an ![$$ {{\left {Fe{\left( {CN} \right)}_{6} } \right]}^{{3 - }} } \mathord{\left/ {\vphantom {{{\left {Fe{\left( {CN} \right)}_{6} } \right]}^{{3 - }} } {{\left {Fe{\left( {CN} \right)}_{6} } \right]}}}} \right. \kern-\nulldelimiterspace} {{\left {Fe{\left( {CN} \right)}_{6} } \right]}}^{{4 - }} $$](/content/ew575h76q3146l25/216_2006_1065_ArticleIEq1.gif) redox mediating system, which allowed amperometric detection of an analytical signal at +400-mV potential. The response time
of the biosensor was less than 1 min. The biosensor showed a linear dependence of the signal vs. creatine concentration at
physiological creatine concentration levels. The optimal pH in 0.1 M tris(hydroxymethyl)aminomethane (Tris)–HCl buffer was
found to be at pH 8.0. The half-life of the biosensor was 8 days in 0.1 M Tris–HCl buffer (pH 8.0) at 20 °C.
Principal scheme of consecutively followed catalytic reactions used to design a biosensor for the determination of creatine |
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| Keywords: | Sarcosine Creatine Creatinine Amperometric Biosensor |
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