Poly(dimethylsiloxane) microchip capillary electrophoresis with electrochemical detection for rapid measurement of acetaminophen and its hydrolysate |
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Authors: | F Y He A L Liu X H Xia |
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Institution: | (1) The State Key Laboratory of Coordination Chemistry, Institute of Analytical Science, Department of Chemistry, Nanjing University, Nanjing, 210093, China |
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Abstract: | Poly(dimethylsiloxane) microchip capillary electrophoresis with amperometric detection has been used for rapid separation and determination of acetaminophen and its hydrolysate, i.e. p-aminophenol. A Pt ultramicroelectrode with a diameter of 10 m positioned at the outlet of the separation channel was used as a working electrode for amperometric detection. Factors influencing separation and detection were investigated and optimized. Results show that acetaminophen and p-aminophenol can be well separated within 35 s with RSD<1% for migration time and <7% for detection current for both analytes. Detection limits for both analytes are estimated to be 5.0 mol L–1 (approximately 0.1 fmol) at S/N=3. This method has been successfully applied to the detection of traces of p-aminophenol in paracetamol tablets. |
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Keywords: | Poly(dimethylsiloxane) Microchip capillary electrophoresis Electrochemical detection Acetaminophen p-Aminophenol Ultramicroelectrode Paracetamol tablet |
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