Effect of D-allose on prostate cancer cell lines: phospholipid profiling by nanoflow liquid chromatography-tandem mass spectrometry |
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Authors: | Jeong Rae Ung Lim Sangsoo Kim Myoung Ok Moon Myeong Hee |
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Institution: | (1) Department of Chemistry, Yonsei University, Seoul, 120-749, South Korea;(2) Division of Life Science and Applied Life Science, Gyeongsang National University, Jinju, 660-701, South Korea; |
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Abstract: | d-Allose, a rare, naturally occurring monosaccharide, is known to exert anti-proliferative effects on cancer cells. The effects
of d-allose on the cellular membranes of hormone-refractory prostate cancer cell line (DU145), hormone-sensitive prostate cancer
cell line (LNCaP), and normal prostate epithelial cells (PrEC) were studied at the molecular level by phospholipid (PL) profiling
using a shotgun lipidomic method. The molecular structures of 85 PL species including 23 phosphatidylcholines, 12 phosphatidylethanolamines
(PEs), 11 phosphatidylserines (PSs), 16 phosphatidylinositols, 9 phosphatidic acids (PAs), and 14 phosphatidylglycerols (PGs)
were identified by data-dependent collision-induced dissociation of nanoflow liquid chromatography–tandem mass spectrometry,
and the PL amounts were quantified. The addition of d-allose to prostate cancer cell lines during their growth phases had negligible or decreased effects on the relative regulation
of PL species, but several new PS molecules (two for DU145 and three for LNCaP) emerged. In contrast, experiments on the PrEC
cell line revealed that some high abundant species (14:0/14:0-PE, 16:2/16:0-PG, and 20:6/18:1-PA) showed significant increases
in concentration. These findings support a mechanism for the anti-proliferative effect of d-allose on prostate cancer cell lines that involves the induction of programmed cell death since PS molecules are known to
induce apoptosis. Principal component analysis was carried out to examine differences in PL distributions among the three
cell lines promoted by d-allose. |
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