Spectrofluorometric determination of nicardipine,nifedipine and isradipine in pharmaceutical preparations and biological fluids |
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Authors: | Sheikha M Al-Ghannam Abeer M Al-Olyan |
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Institution: | (1) Girls College of Science, Department of Chemistry, P.O.Box 838, Dammam-31113, Saudi Arabia |
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Abstract: | A simple and highly sensitive spectrofluorometric method was developed for the determination of some 1,4-dihydropyridine compounds
namely, nicardipine, nifedipine and isradipine in pharmaceutical preparations and biological fluids. The method is based on
the reduction of nicardipine, nifedipine and isradipine with Zn/HCl and measuring the fluorescence intensity obtained (λem/λex) at 460/364, 450/393 and 446/360 nm, respectively. The factors affecting the development of the fluorophore and its stability
were studied and optimized. The effect of some surfactants such as β-cyclodextrin (βCD), carboxymethylcelullose (CMC), sodium
dodecyl sulphate (SDS) and triton X-100, on the fluorescence intensity was studied. The fluorescence intensity-concentration
plots of nicardipine, nifedipine and isradipine were rectilinear over the ranges 0.4–6.0, 0.2–4.0 and 0.1–9.0 μg ml−1 with detection limits of 0.0028, 0.017 and 0.016 μg ml−1, respectively. The proposed method was successfully applied to commercial tablets containing the compounds; the percentage
recovery agreed well with those obtained using the official methods. The method was further extended to the in vitro determination
of the compounds in spiked human plasma and urine samples. A proposal of the reduction reaction pathway was postulated.
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Keywords: | Spectrofluorometric determination Pharmaceutical preparations Biological fluids |
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